Anti-FAS-L FASLG Antibody

Product Name

Anti-FAS-L FASLG Antibody

View all Fas Ligand/TNFSF6 Antibodies

SKU/Catalog Number

A00925-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-FAS-L FASLG Antibody catalog # A00925-1. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FAS-L FASLG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00925-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human FAS-L

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00925-1 is reactive to FASLG in Human, Mouse

Applications

A00925-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

31.485kDa

Background of Fas Ligand/TNFSF6

Links covalently the heme group to the apoprotein of cytochrome c By similarity.

Schaefer L., Genomics 34:166-172(1996).
van den Veyver I.B., Am. J. Med. Genet. 78:179-181(1998).
The MGC Project Team; Genome Res. 14:2121-2127(2004)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of FASLG using Anti-FAS-L FASLG Antibody (A00925-1).

Immunohistochemical analysis of human-colon tissue. Anti-FAS-L at 1:200 (4°C

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Figure 3. Immunohistochemistry validation of FASLG using Anti-FAS-L FASLG Antibody (A00925-1).

Immunohistochemical analysis of human liver cancer tissue. Anti-FAS-L at 1:200 (4°C

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Figure 4. Immunohistochemistry validation of FASLG using Anti-FAS-L FASLG Antibody (A00925-1).

Immunohistochemical analysis of human stomach cancer tissue. Anti-FAS-L at 1:200 (4°C

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Figure 5. Immunohistochemistry validation of FASLG using Anti-FAS-L FASLG Antibody (A00925-1).

Immunohistochemical analysis of mouse colon tissue. Anti-FAS-L at 1:200 (4°C

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Figure 1. Western blotting validation for Anti-FAS-L FASLG Antibody A00925-1

Western blot (WB) analysis of FAS-L polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For FASLG (Source: Uniprot.org, NCBI)

Alternative Names

apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1CD95L; APTL; CD178 antigen; CD178; CD95L; CD95-L; Fas antigen ligand; Fas ligand (TNF superfamily, member 6); Fas Ligand; FASLCD95 ligand; FASLG; TNFSF6; TNFSF6FasL; tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6 FASLG ALPS1B, APT1LG1, APTL, CD178, CD95-L, CD95L, FASL, TNFSF6, TNLG1A Fas ligand tumor necrosis factor ligand superfamily member 6|CD95 ligand|Fas ligand (TNF superfamily, member 6)|apoptosis (APO-1) antigen ligand 1|apoptosis antigen ligand|fas antigen ligand|mutant tumor necrosis factor family member 6|tumor necrosis factor ligand 1A

For more info on FASLG, check out the FASLG Infographic

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In this infographic, you will see the following information for FASLG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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