Anti-FASN/Fatty Acid Synthase Rabbit Monoclonal Antibody

Fatty Acid Synthase/FASN antibody

Boster Bio Anti-FASN/Fatty Acid Synthase Rabbit Monoclonal Antibody catalog # M00941. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00941
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Product Name

Anti-FASN/Fatty Acid Synthase Rabbit Monoclonal Antibody

View all Fatty Acid Synthase/FASN Antibodies

SKU/Catalog Number

M00941

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-FASN/Fatty Acid Synthase Rabbit Monoclonal Antibody catalog # M00941. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FASN/Fatty Acid Synthase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00941)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

IIA-6

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human FASN

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00941 is reactive to FASN in Human, Mouse, Rat

Applications

M00941 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

33 kDa

Calculated molecular weight

273.427kDa

Background of Fatty Acid Synthase/FASN

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For FASN (Source: Uniprot.org, NCBI)

Gene Name

FASN

Full Name

Fatty acid synthase

Weight

273.427kDa

Alternative Names

EC 2.3.1; EC 2.3.1.85; EC 6.3.3.1; FASN; FASshort chain dehydrogenase/reductase family 27X, member 1; Fatty Acid Synthase; MGC14367; MGC15706; OA-519; SDR27X1 FASN FAS, OA-519, SDR27X1 fatty acid synthase fatty acid synthase|short chain dehydrogenase/reductase family 27X, member 1|type I fatty acid synthase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on FASN, check out the FASN Infographic

FASN infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FASN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-FASN/Fatty Acid Synthase Rabbit Monoclonal Antibody

Question

My colleagues were well pleased with the WB result of your anti-FASN/Fatty Acid Synthase Rabbit Monoclonal antibody. However we have seen positive staining in liver cytoplasm. using this antibody. Is that expected? Could you tell me where is FASN supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

Based on literature, liver does express FASN. Generally FASN expresses in cytoplasm. Regarding which tissues have FASN expression, here are a few articles citing expression in various tissues:
B-cell lymphoma, Pubmed ID: 8022791
Brain, Pubmed ID: 7567999, 16625196
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065

Boster Scientific Support

Answered: 2020-04-29

Question

We are currently using anti-FASN/Fatty Acid Synthase Rabbit Monoclonal antibody M00941 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

K. Jones

Verified customer

Asked: 2020-01-08

Answer

The anti-FASN/Fatty Acid Synthase Rabbit Monoclonal antibody (M00941) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-08

Question

We have been able to see staining in rat cervix carcinoma. Do you have any suggestions? Is anti-FASN/Fatty Acid Synthase Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?

Verified Customer

Verified customer

Asked: 2018-02-16

Answer

According to literature cervix carcinoma does express FASN. According to Uniprot.org, FASN is expressed in liver, brain, eye, b-cell lymphoma, lymphoblast, melanoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have FASN expression, here are a few articles citing expression in various tissues:
B-cell lymphoma, Pubmed ID: 8022791
Brain, Pubmed ID: 7567999, 16625196
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065

Boster Scientific Support

Answered: 2018-02-16

Question

My question regards using your anti-FASN/Fatty Acid Synthase Rabbit Monoclonal antibody for activation of gene expression by srebf (srebp) studies. Has this antibody been tested with western blotting on a549 cell lysate? We would like to see some validation images before ordering.

A. Anderson

Verified customer

Asked: 2014-07-21

Answer

I appreciate your inquiry. This M00941 anti-FASN/Fatty Acid Synthase Rabbit Monoclonal antibody is tested on a549 cell lysate. It is guaranteed to work for IP, IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2014-07-21

Question

We ordered your anti-FASN/Fatty Acid Synthase Rabbit Monoclonal antibody for IF on eye last year. I am using rat, and I plan to use the antibody for IP next. We need examining eye as well as b-cell lymphoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?

N. Lewis

Verified customer

Asked: 2014-07-09

Answer

I have checked the website and datasheets of our anti-FASN/Fatty Acid Synthase Rabbit Monoclonal antibody and it seems that M00941 has been validated on rat in both IF and IP. Thus M00941 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2014-07-09

Order DetailsPrice
M00941

100μL

$370
M00941-30ul

30μL sample

$99
M00941-carrier-free

Carrier Free

$370

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