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Product Info Summary
| SKU: | M00941 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-FASN/Fatty Acid Synthase Rabbit Monoclonal Antibody
SKU/Catalog Number
M00941
BM4865 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FASN/Fatty Acid Synthase Rabbit Monoclonal Antibody catalog # M00941. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FASN/Fatty Acid Synthase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00941)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IIA-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FASN
Reactive Species
M00941 is reactive to FASN in Human, Mouse, Rat
Observed Molecular Weight
273 kDa
Calculated molecular weight
273.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00941 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of FASN expression in A549 cell lysate.
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
Specific Publications For Anti-FASN/Fatty Acid Synthase Rabbit Monoclonal Antibody (M00941)
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5 Customer Q&As for Anti-FASN/Fatty Acid Synthase Rabbit Monoclonal Antibody
Question
My colleagues were well pleased with the WB result of your anti-FASN/Fatty Acid Synthase Rabbit Monoclonal antibody. However we have seen positive staining in liver cytoplasm. using this antibody. Is that expected? Could you tell me where is FASN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
Based on literature, liver does express FASN. Generally FASN expresses in cytoplasm. Regarding which tissues have FASN expression, here are a few articles citing expression in various tissues:
B-cell lymphoma, Pubmed ID: 8022791
Brain, Pubmed ID: 7567999, 16625196
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Boster Scientific Support
Answered: 2020-04-29
Question
We are currently using anti-FASN/Fatty Acid Synthase Rabbit Monoclonal antibody M00941 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
K. Jones
Verified customer
Asked: 2020-01-08
Answer
The anti-FASN/Fatty Acid Synthase Rabbit Monoclonal antibody (M00941) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-08
Question
We have been able to see staining in rat cervix carcinoma. Do you have any suggestions? Is anti-FASN/Fatty Acid Synthase Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2018-02-16
Answer
According to literature cervix carcinoma does express FASN. According to Uniprot.org, FASN is expressed in liver, brain, eye, b-cell lymphoma, lymphoblast, melanoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have FASN expression, here are a few articles citing expression in various tissues:
B-cell lymphoma, Pubmed ID: 8022791
Brain, Pubmed ID: 7567999, 16625196
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Boster Scientific Support
Answered: 2018-02-16
Question
My question regards using your anti-FASN/Fatty Acid Synthase Rabbit Monoclonal antibody for activation of gene expression by srebf (srebp) studies. Has this antibody been tested with western blotting on a549 cell lysate? We would like to see some validation images before ordering.
A. Anderson
Verified customer
Asked: 2014-07-21
Answer
I appreciate your inquiry. This M00941 anti-FASN/Fatty Acid Synthase Rabbit Monoclonal antibody is tested on a549 cell lysate. It is guaranteed to work for IP, IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-07-21
Question
We ordered your anti-FASN/Fatty Acid Synthase Rabbit Monoclonal antibody for IF on eye last year. I am using rat, and I plan to use the antibody for IP next. We need examining eye as well as b-cell lymphoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
N. Lewis
Verified customer
Asked: 2014-07-09
Answer
I have checked the website and datasheets of our anti-FASN/Fatty Acid Synthase Rabbit Monoclonal antibody and it seems that M00941 has been validated on rat in both IF and IP. Thus M00941 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-07-09
