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Product Info Summary
| SKU: | M02401 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Ferritin FTH1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02401
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Ferritin FTH1 Rabbit Monoclonal Antibody catalog # M02401. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ferritin FTH1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02401)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FBA-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Ferritin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02401 is reactive to FTH1 in Human, Mouse, Rat
Applications
M02401 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
69 kDa
Calculated molecular weight
21.226kDa
Background of FTH1
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of Jurkat cells, using Ferritin Antibody.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Western blot analysis of Ferritin expression in Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For FTH1 (Source: Uniprot.org, NCBI)
Gene Name
FTH1
Full Name
Ferritin heavy chain
Weight
21.226kDa
Superfamily
ferritin family
Alternative Names
apoferritin; Cell proliferation-inducing gene 15 protein; Ferritin H subunit; ferritin heavy chain; ferritin, heavy polypeptide 1; FHC; FTHEC 1.16.3.1; FTHL6MGC104426; PIG15; placenta immunoregulatory factor; PLIF; proliferation-inducing protein 15 FTH1 FHC, FTH, FTHL6, HFE5, PIG15, PLIF ferritin heavy chain 1 ferritin heavy chain|apoferritin|cell proliferation-inducing gene 15 protein|ferritin H subunit|ferritin, heavy polypeptide 1|placenta immunoregulatory factor|proliferation-inducing protein 15
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FTH1, check out the FTH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FTH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ferritin FTH1 Rabbit Monoclonal Antibody (M02401)
Hello CJ!
M02401 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xiao J,Zhang S,Tu B,Jiang X,Cheng S,Tang Q,Zhang J,Qin X,Wang B,Zou Z,Chen C. Arsenite induces ferroptosis in the neuronal cells via activation of ferritinophagy. Food Chem Toxicol.2021 Mar 12:112114.doi:10.1016/j.fct.2021.112114.Epub ahead of print.PMID:33722599.
Species: Mouse,Rat
M02401 usage in article: APP:WB, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION: 1:200
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Ferritin FTH1 Rabbit Monoclonal Antibody
Question
I have a question about product M02401, anti-Ferritin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02401 anti-Ferritin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-05
Question
I was wanting to use your anti-Ferritin Rabbit Monoclonal antibody for ICC for mouse tibial nerve on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse tibial nerve identification?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
You can see on the product datasheet, M02401 anti-Ferritin Rabbit Monoclonal antibody has been validated for IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse tibial nerve in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-21
Question
Does M02401 anti-Ferritin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-07
Answer
As indicated on the product datasheet, M02401 anti-Ferritin Rabbit Monoclonal antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-07
Question
I see that the anti-Ferritin Rabbit Monoclonal antibody M02401 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-12-12
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-12-12
Question
Will anti-Ferritin Rabbit Monoclonal antibody M02401 work for ICC with tibial nerve?
P. Krishna
Verified customer
Asked: 2018-01-16
Answer
According to the expression profile of tibial nerve, FTH1 is highly expressed in tibial nerve. So, it is likely that anti-Ferritin Rabbit Monoclonal antibody M02401 will work for ICC with tibial nerve.
Boster Scientific Support
Answered: 2018-01-16
Question
Is there a BSA free version of anti-Ferritin Rabbit Monoclonal antibody M02401 available?
L. Dhar
Verified customer
Asked: 2017-09-25
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Ferritin Rabbit Monoclonal antibody M02401 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-25
Question
We are currently using anti-Ferritin Rabbit Monoclonal antibody M02401 for rat tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-24
Answer
The anti-Ferritin Rabbit Monoclonal antibody (M02401) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-24
