Anti-FSH beta/FSHB Antibody Picoband™

Product Name

Anti-FSH beta/FSHB Antibody Picoband™

View all FSH beta Antibodies

SKU/Catalog Number

PB9106

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FSH beta/FSHB Antibody Picoband™ catalog # PB9106. Tested in IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FSH beta/FSHB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9106)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human FSH beta recombinant protein (Position: N19-E129). Human FSH beta shares 90% and 89% amino acid (aa) sequences identity with mouse and rat FSH beta, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9106 is reactive to FSHB in Human, Mouse

Applications

PB9106 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

36 kDa

Calculated molecular weight

14.7kDa

Background of FSH beta

FSHB, also known as Follitropin subunit beta, is a protein that in humans is encoded by the FSHB gene, and this gene is mapped to 11p14.1. FSHB enables ovarian folliculogenesis to the antral follicle stage and is essential for Sertoli cell proliferation and maintenance of sperm quality in the testis. It can stimulate development of follicle and spermatogenesis in the reproductive organs. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of FSH beta using anti-FSH beta antibody (PB9106).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours
Lane 1: recombinant human FSH beta protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSH beta antigen affinity purified polyclonal antibody (Catalog # PB9106) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSH beta at approximately 36 kDa. The expected band size for FSH beta is at 36 kDa.

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Figure 2. IHC analysis of FSH beta using anti-FSH beta antibody (PB9106).
FSH beta was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FSH beta Antibody (PB9106) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of FSH beta using anti-FSH beta antibody (PB9106).
FSH beta was detected in a paraffin-embedded section of human mammry cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FSH beta Antibody (PB9106) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For FSHB (Source: Uniprot.org, NCBI)

Alternative Names

follicle stimulating hormone, beta polypeptide; Follicle-stimulating hormone beta subunit; Follitropin beta chain; follitropin subunit beta; Follitropin; follitropin, beta chain; FSH beta; FSHB; FSH-B; FSH-beta FSHB HH24 follicle stimulating hormone subunit beta follitropin subunit beta|FSH-B|FSH-beta|follicle stimulating hormone beta subunit|follicle stimulating hormone, beta polypeptide|follitropin, beta chain

For more info on FSHB, check out the FSHB Infographic

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In this infographic, you will see the following information for FSHB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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