Anti-GAP43 Antibody

Boster Bio Anti-GAP43 Antibody catalog # M01868-4. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Human, Mouse, Pig, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: M01868-4
Size: 100ul
Reactive Species: Bovine, Chicken, Equine, Human, Mouse, Pig, Rat
Host: Chicken
Application: IF, IHC, WB

Product Name

Anti-GAP43 Antibody

See all GAP-43 products

SKU/Catalog Number

M01868-4

Size

100ul

Description

Boster Bio Anti-GAP43 Antibody catalog # M01868-4. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Human, Mouse, Pig, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GAP43 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01868-4)

Host

Chicken

Contents

Supplied as an aliquot of IgY preparation plus 5mM NaN3

Clonality

Polyclonal

Immunogen

C-terminal peptide of rodent GAP43, KEDPEADQEHA coupled to KLH

Reactive Species

M01868-4 is reactive to GAP43 in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat

Applications

M01868-4 is guaranteed for IF, IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB: 1:1,000-1-:5,000. IF/IHC: 1:500-1:1,000

Validation Images & Assay Conditions

Gene/Protein Information For GAP43 (Source: Uniprot.Org, NCBI)

Uniprot ID

P17677

Gene ID

2596

Gene Name

GAP43

Full Name

Neuromodulin

Weight

24.803kDa

Superfamily

neuromodulin family

Alternative Names

Axonal membrane protein GAP-43; B-50; Basp2; calmodulin-binding protein P-57; GAP43; GAP-43; growth associated protein 43; Growth-associated protein 43; nerve growth-related peptide GAP43; Neural phosphoprotein B-50; Neuromodulin; neuron growth-associated protein 43; PP46; protein F1 GAP43 B-50, GAP-43, PP46 growth associated protein 43 neuromodulin|axonal membrane protein GAP-43|calmodulin-binding protein P-57|nerve growth-related peptide GAP43|neural phosphoprotein B-50|neuron growth-associated protein 43|protein F1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GAP43, check out the GAP43 Infographic

GAP43 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GAP43: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M01868-4 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Liu Z, Liu H, Zeng ZH. Biomed Res Int. 2018 Mar 7;2018:8697913. doi: 10.1155/2018/8697913. eCollection 2018. Chronic Unpredictable Mild Stress Causing Cardiac and Thoracic Spinal Cord Electrophysiological Abnormalities May Be Associated with Incre...

Liu H, Lv P, Zhu Y, Wu H, Zhang K, Xu F, Zheng L, Zhao J. Sci Rep. 2017 Jan 5;7:39869. doi: 10.1038/srep39869. Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo

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16 Customer Q&As for Anti-GAP43 Antibody

Question

Is this M01868-4 anti-GAP43 antibody reactive to the isotypes of GAP43?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

The immunogen of M01868-4 anti-GAP43 antibody is C-terminal peptide of rodent GAP43, KEDPEADQEHA coupled to KLH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-23

Question

I see that the anti-GAP43 antibody M01868-4 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-03-16

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-16

Question

We have observed staining in mouse brain cortex subthalamic nucleus. Do you have any suggestions? Is anti-GAP43 antibody supposed to stain brain cortex subthalamic nucleus positively?

Verified Customer

Verified customer

Asked: 2020-02-05

Answer

According to literature brain cortex subthalamic nucleus does express GAP43. According to Uniprot.org, GAP43 is expressed in anterior cingulate cortex, brain cortex subthalamic nucleus, brain, among other tissues. Regarding which tissues have GAP43 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Brain cortex, and Subthalamic nucleus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-02-05

Question

See attached the WB image, lot number and protocol we used for anterior cingulate cortex using anti-GAP43 antibody M01868-4. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-16

Question

My team were content with the WB result of your anti-GAP43 antibody. However we have been able to see positive staining in anterior cingulate cortex cell membrane using this antibody. Is that expected? Could you tell me where is GAP43 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

According to literature, anterior cingulate cortex does express GAP43. Generally GAP43 expresses in cell membrane. Regarding which tissues have GAP43 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Brain cortex, and Subthalamic nucleus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-10-15

Question

Does M01868-4 anti-GAP43 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

S. Baker

Verified customer

Asked: 2019-08-16

Answer

As indicated on the product datasheet, M01868-4 anti-GAP43 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-16

Question

I was wanting to use your anti-GAP43 antibody for WB for bovine anterior cingulate cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for bovine anterior cingulate cortex identification?

Verified Customer

Verified customer

Asked: 2019-07-29

Answer

It shows on the product datasheet, M01868-4 anti-GAP43 antibody has been validated for IF, IHC, WB on bovine, chicken, equine, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in bovine anterior cingulate cortex in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-29

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for anterior cingulate cortex using anti-GAP43 antibody M01868-4. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-07-18

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-18

Question

My question regarding product M01868-4, anti-GAP43 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-02-07

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01868-4 anti-GAP43 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-02-07

Question

Is a blocking peptide available for product anti-GAP43 antibody (M01868-4)?

Verified Customer

Verified customer

Asked: 2019-01-11

Answer

We do provide the blocking peptide for product anti-GAP43 antibody (M01868-4). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-01-11

Question

We are interested in using your anti-GAP43 antibody for regulation of filopodium assembly studies. Has this antibody been tested with western blotting on c6 cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-11-14

Answer

I appreciate your inquiry. This M01868-4 anti-GAP43 antibody is tested on mouse brain, c6 cells. It is guaranteed to work for IF, IHC, WB in bovine, chicken, equine, human, mouse, pig, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-11-14

Question

We are interested in to test anti-GAP43 antibody M01868-4 on bovine anterior cingulate cortex for research purposes, then I may be interested in using anti-GAP43 antibody M01868-4 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

H. Dhar

Verified customer

Asked: 2018-08-23

Answer

The products we sell, including anti-GAP43 antibody M01868-4, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-08-23

Question

Do you have a BSA free version of anti-GAP43 antibody M01868-4 available?

Verified Customer

Verified customer

Asked: 2018-08-01

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-GAP43 antibody M01868-4 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-08-01

Question

We bought anti-GAP43 antibody for WB on anterior cingulate cortex a few years ago. I am using rat, and I plan to use the antibody for IF next. We want examining anterior cingulate cortex as well as brain in our next experiment. Do you have any suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2018-05-07

Answer

I took a look at the website and datasheets of our anti-GAP43 antibody and it appears that M01868-4 has been validated on rat in both WB and IF. Thus M01868-4 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-05-07

Question

Does anti-GAP43 antibody M01868-4 work for WB with anterior cingulate cortex?

T. Singh

Verified customer

Asked: 2018-02-16

Answer

According to the expression profile of anterior cingulate cortex, GAP43 is highly expressed in anterior cingulate cortex. So, it is likely that anti-GAP43 antibody M01868-4 will work for WB with anterior cingulate cortex.

Boster Scientific Support

Answered: 2018-02-16

Question

We are currently using anti-GAP43 antibody M01868-4 for pig tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says bovine, chicken, equine, human, mouse, pig, rat. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2017-07-10

Answer

The anti-GAP43 antibody (M01868-4) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-07-10

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M01868-4
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