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Product Info Summary
| SKU: | M01868 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GAP43 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01868
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GAP43 Rabbit Monoclonal Antibody catalog # M01868. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GAP43 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01868)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FOG-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GAP43
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01868 is reactive to GAP43 in Human, Mouse, Rat
Applications
M01868 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
24.803kDa
Background of GAP-43
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:10000-1:50000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
FC 1:20
Validation Images & Assay Conditions
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Western blot analysis of GAP43 expression in SH-SY5Y cell lysate.
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Immunohistochemical analysis of paraffin-embedded human stomach, using GAP43 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For GAP43 (Source: Uniprot.org, NCBI)
Gene Name
GAP43
Full Name
Neuromodulin
Weight
24.803kDa
Superfamily
neuromodulin family
Alternative Names
Axonal membrane protein GAP-43; B-50; Basp2; calmodulin-binding protein P-57; GAP43; GAP-43; growth associated protein 43; Growth-associated protein 43; nerve growth-related peptide GAP43; Neural phosphoprotein B-50; Neuromodulin; neuron growth-associated protein 43; PP46; protein F1 GAP43 B-50, GAP-43, PP46 growth associated protein 43 neuromodulin|axonal membrane protein GAP-43|calmodulin-binding protein P-57|nerve growth-related peptide GAP43|neural phosphoprotein B-50|neuron growth-associated protein 43|protein F1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GAP43, check out the GAP43 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GAP43: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GAP43 Rabbit Monoclonal Antibody (M01868)
Hello CJ!
M01868 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Fructus Corni extract-induced neuritogenesis in PC12 cells is associated with the suppression of stromal interaction molecule 1 expression and inhibition of Ca2+ influx
Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats
Liu Z, Liu H, Zeng ZH. Biomed Res Int. 2018 Mar 7;2018:8697913. doi: 10.1155/2018/8697913. eCollection 2018. Chronic Unpredictable Mild Stress Causing Cardiac and Thoracic Spinal Cord Electrophysiological Abnormalities May Be Associated with Incre...
Liu H, Lv P, Zhu Y, Wu H, Zhang K, Xu F, Zheng L, Zhao J. Sci Rep. 2017 Jan 5;7:39869. doi: 10.1038/srep39869. Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo
Recommended Resources
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-GAP43 Rabbit Monoclonal Antibody
Question
Our team were content with the WB result of your anti-GAP43 Rabbit Monoclonal antibody. However we have been able to see positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is GAP43 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
According to literature, brain does express GAP43. Generally GAP43 expresses in cell membrane. Regarding which tissues have GAP43 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Brain cortex, and Subthalamic nucleus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-04-09
Question
We are currently using anti-GAP43 Rabbit Monoclonal antibody M01868 for mouse tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
The anti-GAP43 Rabbit Monoclonal antibody (M01868) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-13
Question
We ordered your anti-GAP43 Rabbit Monoclonal antibody for IF on brain in a previous project. I am using human, and We intend to use the antibody for WB next. I am looking for examining brain as well as brain cortex subthalamic nucleus in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
I looked at the website and datasheets of our anti-GAP43 Rabbit Monoclonal antibody and it seems that M01868 has been validated on human in both IF and WB. Thus M01868 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-05-30
Question
We have been able to see staining in mouse brain. Are there any suggestions? Is anti-GAP43 Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2017-07-07
Answer
Based on literature brain does express GAP43. Based on Uniprot.org, GAP43 is expressed in anterior cingulate cortex, brain cortex subthalamic nucleus, brain, among other tissues. Regarding which tissues have GAP43 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Brain cortex, and Subthalamic nucleus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-07-07
