Anti-GAPDH Antibody

GAPDH antibody

Boster Bio Anti-GAPDH Antibody catalog # M00227-4. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Human, Mouse, Pig, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: M00227-4
Size: 100ul
Reactive Species: Bovine, Chicken, Equine, Human, Mouse, Pig, Rat
Host: Rabbit
Application: IF, IHC, WB

Customers Who Bought This Also Bought

Product Name

Anti-GAPDH Antibody

View all GAPDH Antibodies

SKU/Catalog Number

M00227-4

Size

100ul

Description

Boster Bio Anti-GAPDH Antibody catalog # M00227-4. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Human, Mouse, Pig, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GAPDH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227-4)

Host

Rabbit

Contents

Antibody is supplied as an aliquot of serum

Clonality

Polyclonal

Immunogen

Native porcine purified protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00227-4 is reactive to GAPDH in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat

Applications

M00227-4 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

36.053kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:30,000 IF/ICC and IHC: 1:10,000

Validation Images & Assay Conditions

Gene/Protein Information For GAPDH (Source: Uniprot.org, NCBI)

Gene Name

GAPDH

Full Name

Glyceraldehyde-3-phosphate dehydrogenase

Weight

36.053kDa

Superfamily

glyceraldehyde-3-phosphate dehydrogenase family

Alternative Names

1D4 GAPDH; aging-associated gene 9 protein; Cytoplasm Marker; EC 1.2.1; EC 1.2.1.12; EC 2.6.99.-; G3PD; G3PD; G3PDH; GAPD; GAPDH; GAPDPeptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38; OCT1 coactivator in S phase; Peptidyl-cysteine S-nitrosylase GAPDH GAPDH G3PD, GAPD, HEL-S-162eP glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase|OCAS, p38 component|Oct1 coactivator in S phase, 38 Kd component|aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GAPDH, check out the GAPDH Infographic

GAPDH infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GAPDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00227-4 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ma Y,Chai H,Ding Q,Qian Q,Yan Z,Ding B,Dou X,Li S.Hepatic SIRT3 Upregulation in Response to Chronic Alcohol Consumption Contributes to Alcoholic Liver Disease in Mice.Front Physiol.2019 Aug 13;10:1042.doi:10.3389/fphys.2019.01042.PMID:31474877;PMCID:PMC6707764.
Species: Human,Mouse
M00227-4 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA

Lu X,Yang B,Yu H,Hu X,Nie J,Wan B,Zhang M,Lü C.Epigenetic mechanisms underlying the effects of triptolide and tripchlorolide on the expression of neuroligin-1 in the hippocampus of APP/PS1 transgenic mice.Pharm Biol.2019 Dec;57(1):453-459.doi:10. 1080/13880209.2019.1629463.PMID:31311385;PMCID:PMC6691810.
Species: Mouse
M00227-4 usage in article: APP:WB, SAMPLE:HIPPOCAMPAL TISSUE, DILUTION:1:1000

Wei X,Bao Y,Zhan X,Zhang L,Hao G,Zhou J,Chen Q.MiR-200a ameliorates peritoneal fibrosis and functional deterioration in a rat model of peritoneal dialysis.Int Urol Nephrol.2019 May;51(5):889-896.doi:10.1007/s11255-019-02122-4.Epub 2019 Mar 19.PMID:30888602;PMCID:PMC6499761.
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:PERITONEAL TISSUE, DILUTION:NA

Li,Z.,Wu,Z.,Yan,J.et al.Gut microbe-derived metabolite trimethylamine N-oxide induces cardiac hypertrophy and fibrosis.Lab Invest 99,346–357(2019).https://doi.org/10.1038/s41374-018-0091-y
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:CARDIOMYOCYTES, DILUTION:1:10000

Zhao,Y.,Jia,W.,Ren,S.,Xiao,W.,Li,G.,Jin,L.,& Lin,Y.(2021). Difluoromethylornithine attenuates isoproterenol‑induced cardiac hypertrophy by regulating apoptosis, autophagy and the mitochondria‑associated membranes pathway.Experimental and Therapeutic Medicine,22,870.https://doi.org/10.3892/etm.2021.10302
Species: Rat

Tang,K.,Zhang,W.S.,Liu,Y.,Zhu,W.,& Qiu,J.(2021).Physicochemical properties and in vitro osteocompatibility of different titanium surfaces stored in a saline solution.Materials Research Express.
Species: Mouse
M00227-4 usage in article: APP:WB, SAMPLE:MC3T3-E1 CELL, DILUTION:NA

Huang X,Pei W,Ni B,Zhang R,You H.Chondroprotective and antiarthritic effects of Galangin in Osteoarthritis: An in Vitro and in Vivo Study.Eur J Pharmacol.2021 Jun 3:174232.doi:10.1016/j. ejphar.2021. 174232.Epub ahead of print.PMID:34090897.
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION:NA

Deng S,Zhang Y,Liao Z,Huang J,Huang R,Li Z.S100A4 plays a key role in TRPV3 ion channel expression and its electrophysiological function.Neurosci Lett.2021 May 28:135999.doi:10.1016/j.neulet.2021.135999.Epub ahead of print.PMID:34058292.
Species: Human
M00227-4 usage in article: APP:WB, SAMPLE:HEK293 T CELL, DILUTION:NA

Guo H,Zhao W,Liu A,Wu M,Shuai Y,Li B,Huang X,Liu X,Yang X,Guo X,Xuan K,Jin Y.SHED promote angiogenesis in stem cell-mediated dental pulp regeneration.Biochem Biophys Res Commun.2020 Sep 3;529(4):1158-1164.doi:10.1016/j.bbrc.2020.06.151.Epub 2020 Aug 3.PMID:32819580.
Species: Human,Pig
M00227-4 usage in article: APP:WB, SAMPLE:R-SHED AND SHED, DILUTION:NA

Chen M,Lu C,Lu H,Zhang J,Qin D,Liu S,Li X,Zhang L.Farnesoid X receptor via Notch1 directs asymmetric cell division of Sox9+ cells to prevent the development of liver cancer in a mouse model.Stem Cell Res Ther.2021 Apr 12;12(1):232.doi:10.1186/s13287-021-02298-6.PMID:33845903;PMCID:PMC8042944.
Species: Human,Mouse
M00227-4 usage in article: APP:WB, SAMPLE:LIVER TISSUE AND HUH7 CELL, DILUTION:NA

Have you used Anti-GAPDH Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-GAPDH Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

16 Customer Q&As for Anti-GAPDH Antibody

Question

My question regarding product M00227-4, anti-GAPDH antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

C. Rodriguez

Verified customer

Asked: 2020-04-17

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00227-4 anti-GAPDH antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-17

Question

I see that the anti-GAPDH antibody M00227-4 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-02-20

Question

Our lab want to know about using your anti-GAPDH antibody for gluconeogenesis studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-12-09

Answer

Thank you for your inquiry. This M00227-4 anti-GAPDH antibody is validated on hela cells. It is guaranteed to work for IF, IHC, WB in bovine, chicken, equine, human, mouse, pig, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-12-09

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for muscle using anti-GAPDH antibody M00227-4. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-21

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-21

Question

I was wanting to use your anti-GAPDH antibody for WB for pig muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for pig muscle identification?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

You can see on the product datasheet, M00227-4 anti-GAPDH antibody has been tested for IF, IHC, WB on bovine, chicken, equine, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in pig muscle in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-05

Question

Here is the WB image, lot number and protocol we used for muscle using anti-GAPDH antibody M00227-4. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-11-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-27

Question

We are currently using anti-GAPDH antibody M00227-4 for chicken tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says bovine, chicken, equine, human, mouse, pig, rat. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2018-08-27

Answer

The anti-GAPDH antibody (M00227-4) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-08-27

Question

Do you have a BSA free version of anti-GAPDH antibody M00227-4 available?

Verified Customer

Verified customer

Asked: 2018-07-20

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GAPDH antibody M00227-4 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-07-20

Question

Does anti-GAPDH antibody M00227-4 work for WB with muscle?

G. Bhatt

Verified customer

Asked: 2018-01-16

Answer

According to the expression profile of muscle, GAPDH is highly expressed in muscle. So, it is likely that anti-GAPDH antibody M00227-4 will work for WB with muscle.

Boster Scientific Support

Answered: 2018-01-16

Question

Is this M00227-4 anti-GAPDH antibody reactive to the isotypes of GAPDH?

Verified Customer

Verified customer

Asked: 2017-11-06

Answer

The immunogen of M00227-4 anti-GAPDH antibody is Native porcine purified protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-11-06

Question

Our team were content with the WB result of your anti-GAPDH antibody. However we have observed positive staining in lung cytosol using this antibody. Is that expected? Could you tell me where is GAPDH supposed to be expressed?

H. Taylor

Verified customer

Asked: 2017-10-02

Answer

From what I have seen in literature, lung does express GAPDH. Generally GAPDH expresses in cytoplasm, cytosol. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305

Boster Scientific Support

Answered: 2017-10-02

Question

We bought anti-GAPDH antibody for IF on lung in a previous project. I am using bovine, and I plan to use the antibody for WB next. We want examining lung as well as colon adenocarcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2017-09-20

Answer

I looked at the website and datasheets of our anti-GAPDH antibody and it seems that M00227-4 has been tested on bovine in both IF and WB. Thus M00227-4 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in bovine even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-09-20

Question

We have been able to see staining in mouse colon adenocarcinoma. Do you have any suggestions? Is anti-GAPDH antibody supposed to stain colon adenocarcinoma positively?

R. Johnson

Verified customer

Asked: 2016-11-07

Answer

From literature colon adenocarcinoma does express GAPDH. From Uniprot.org, GAPDH is expressed in smooth muscle tissue, liver, lung, placenta, astrocytoma, leukemia, colon adenocarcinoma, eye, kidney, lung, lymph placenta, muscle, platelet, prostatic carcinoma, brain, cajal-retzius cell fetal brain cortex, heart, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305

Boster Scientific Support

Answered: 2016-11-07

Question

Will M00227-4 anti-GAPDH antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

M. Banerjee

Verified customer

Asked: 2015-07-17

Answer

As indicated on the product datasheet, M00227-4 anti-GAPDH antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-07-17

Question

Is a blocking peptide available for product anti-GAPDH antibody (M00227-4)?

L. Williams

Verified customer

Asked: 2014-12-24

Answer

We do provide the blocking peptide for product anti-GAPDH antibody (M00227-4). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2014-12-24

Question

I am interested in to test anti-GAPDH antibody M00227-4 on pig muscle for research purposes, then I may be interested in using anti-GAPDH antibody M00227-4 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

V. Jackson

Verified customer

Asked: 2014-02-12

Answer

The products we sell, including anti-GAPDH antibody M00227-4, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-02-12

$299
Backordered.

Lead time for this item is typically 4-5 weeks

Get A Quote
Eddy test
In stock
Order Product
M00227-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.