Anti-GAPDH Antibody

Boster Bio Anti-GAPDH Antibody catalog # M00227-4. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Human, Mouse, Pig, Rat. Cited in 132 publication(s).

Product Info Summary

SKU: M00227-4
Size: 100ul
Reactive Species: Bovine, Chicken, Equine, Human, Mouse, Pig, Rat
Host: Rabbit
Application: IF, IHC, WB

Product Name

Anti-GAPDH Antibody

See all Gapdh products

SKU/Catalog Number

M00227-4

Size

100ul

Description

Boster Bio Anti-GAPDH Antibody catalog # M00227-4. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Human, Mouse, Pig, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GAPDH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227-4)

Host

Rabbit

Contents

Antibody is supplied as an aliquot of serum

Clonality

Polyclonal

Immunogen

Native porcine purified protein

Reactive Species

M00227-4 is reactive to Gapdh in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat

Applications

M00227-4 is guaranteed for IF, IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB: 1:30,000 IF/ICC and IHC: 1:10,000

Validation Images & Assay Conditions

Gene/Protein Information For Gapdh (Source: Uniprot.Org, NCBI)

Uniprot ID

P04797

Gene ID

24383

Gene Name

Gapdh

Full Name

Glyceraldehyde-3-phosphate dehydrogenase

Weight

35.828kDa

Superfamily

glyceraldehyde-3-phosphate dehydrogenase family

Alternative Names

1D4 GAPDH; aging-associated gene 9 protein; Cytoplasm Marker; EC 1.2.1; EC 1.2.1.12; EC 2.6.99.-; G3PD; G3PD; G3PDH; GAPD; GAPDH; GAPDPeptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38; OCT1 coactivator in S phase; Peptidyl-cysteine S-nitrosylase GAPDH Gapdh|BARS-38, Gapd|glyceraldehyde-3-phosphate dehydrogenase|glyceraldehyde-3-phosphate dehydrogenase|38 kDa BFA-dependent ADP-ribosylation substrate|peptidyl-cysteine S-nitrosylase GAPDH

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Gapdh, check out the Gapdh Infographic

Gapdh infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Gapdh: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00227-4 has been cited in 132 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ma Y,Chai H,Ding Q,Qian Q,Yan Z,Ding B,Dou X,Li S.Hepatic SIRT3 Upregulation in Response to Chronic Alcohol Consumption Contributes to Alcoholic Liver Disease in Mice.Front Physiol.2019 Aug 13;10:1042.doi:10.3389/fphys.2019.01042.PMID:31474877;PMCID:PMC6707764.
Species: Human,Mouse
M00227-4 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA

Lu X,Yang B,Yu H,Hu X,Nie J,Wan B,Zhang M,Lü C.Epigenetic mechanisms underlying the effects of triptolide and tripchlorolide on the expression of neuroligin-1 in the hippocampus of APP/PS1 transgenic mice.Pharm Biol.2019 Dec;57(1):453-459.doi:10. 1080/13880209.2019.1629463.PMID:31311385;PMCID:PMC6691810.
Species: Mouse
M00227-4 usage in article: APP:WB, SAMPLE:HIPPOCAMPAL TISSUE, DILUTION:1:1000

Wei X,Bao Y,Zhan X,Zhang L,Hao G,Zhou J,Chen Q.MiR-200a ameliorates peritoneal fibrosis and functional deterioration in a rat model of peritoneal dialysis.Int Urol Nephrol.2019 May;51(5):889-896.doi:10.1007/s11255-019-02122-4.Epub 2019 Mar 19.PMID:30888602;PMCID:PMC6499761.
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:PERITONEAL TISSUE, DILUTION:NA

Li,Z.,Wu,Z.,Yan,J.et al.Gut microbe-derived metabolite trimethylamine N-oxide induces cardiac hypertrophy and fibrosis.Lab Invest 99,346–357(2019).https://doi.org/10.1038/s41374-018-0091-y
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:CARDIOMYOCYTES, DILUTION:1:10000

Zhao,Y.,Jia,W.,Ren,S.,Xiao,W.,Li,G.,Jin,L.,& Lin,Y.(2021). Difluoromethylornithine attenuates isoproterenol‑induced cardiac hypertrophy by regulating apoptosis, autophagy and the mitochondria‑associated membranes pathway.Experimental and Therapeutic Medicine,22,870.https://doi.org/10.3892/etm.2021.10302
Species: Rat

Tang,K.,Zhang,W.S.,Liu,Y.,Zhu,W.,& Qiu,J.(2021).Physicochemical properties and in vitro osteocompatibility of different titanium surfaces stored in a saline solution.Materials Research Express.
Species: Mouse
M00227-4 usage in article: APP:WB, SAMPLE:MC3T3-E1 CELL, DILUTION:NA

Huang X,Pei W,Ni B,Zhang R,You H.Chondroprotective and antiarthritic effects of Galangin in Osteoarthritis: An in Vitro and in Vivo Study.Eur J Pharmacol.2021 Jun 3:174232.doi:10.1016/j. ejphar.2021. 174232.Epub ahead of print.PMID:34090897.
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION:NA

Deng S,Zhang Y,Liao Z,Huang J,Huang R,Li Z.S100A4 plays a key role in TRPV3 ion channel expression and its electrophysiological function.Neurosci Lett.2021 May 28:135999.doi:10.1016/j.neulet.2021.135999.Epub ahead of print.PMID:34058292.
Species: Human
M00227-4 usage in article: APP:WB, SAMPLE:HEK293 T CELL, DILUTION:NA

Guo H,Zhao W,Liu A,Wu M,Shuai Y,Li B,Huang X,Liu X,Yang X,Guo X,Xuan K,Jin Y.SHED promote angiogenesis in stem cell-mediated dental pulp regeneration.Biochem Biophys Res Commun.2020 Sep 3;529(4):1158-1164.doi:10.1016/j.bbrc.2020.06.151.Epub 2020 Aug 3.PMID:32819580.
Species: Human,Pig
M00227-4 usage in article: APP:WB, SAMPLE:R-SHED AND SHED, DILUTION:NA

Chen M,Lu C,Lu H,Zhang J,Qin D,Liu S,Li X,Zhang L.Farnesoid X receptor via Notch1 directs asymmetric cell division of Sox9+ cells to prevent the development of liver cancer in a mouse model.Stem Cell Res Ther.2021 Apr 12;12(1):232.doi:10.1186/s13287-021-02298-6.PMID:33845903;PMCID:PMC8042944.
Species: Human,Mouse
M00227-4 usage in article: APP:WB, SAMPLE:LIVER TISSUE AND HUH7 CELL, DILUTION:NA

Zhu X,Xue D,Liu J,Dong F,Li Y,Liu Y.Nodal is involved in chemoresistance of renal cell carcinoma cells via regulation of ABCB1.J Cancer 2021;12(7):2041-2049.doi:10.7150/jca.52092.Available from https://www.jcancer.org/v12p2041.htm
Species: Human
M00227-4 usage in article: APP:WB, SAMPLE:GRC-1 CELL AND ACHN, DILUTION:1:1000

Zhang Y,Zhou G,Peng Y,Wang M,Li X.Anti-hyperglycemic and anti-hyperlipidemic effects of a special fraction of Luohanguo extract on obese T2DM rats.J Ethnopharmacol.2020 Jan 30;247:112273.doi:10.1016/j.jep.2019.112273.Epub 2019 Oct 3.PMID:31586692.
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA

She Y,Sun J,Hou P,Fang P, Zhang Z. Time-restricted feeding attenuates gluconeogenic activity through inhibition of PGC-1α expression and activity. Physiol Behav. 2021 Mar 15;231:113313. doi: 10.1016/j.physbeh.2021.113313. Epub 2021 Jan 4.PMID:33412190.
Species: Mouse
M00227-4 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA

Wang P,Zhang Y,Wang Z,Yang A,Li Y,Zhang Q.miR-128 regulates epilepsy sensitivity in mice by suppressing SNAP-25 and SYT1 expression in the hippocampus.Biochem Biophys Res Commun.2021 Feb 8;545:195-202.doi:10.1016/j.bbrc.2021.01.079.Epub ahead of print.PMI
Species: Mouse
M00227-4 usage in article: APP:WB, SAMPLE:BRAIN TISSUE AND NEURON CELL, DILUTION:1:2000

Lin X,Chen H,Chen M,Li T,Lai Y,Lin L,Lin P,Liu J,Zhang Y,Chen R,Du H,Jiang X,Liu N.Bone marrow-derived mesenchymal stem cells improve post-ischemia neurological function in rats via the PI3K/AKT/GSK-3β/CRMP-2 pathway.Mol Cell Biochem.2021 Feb 9.doi:10.100
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:500

Gang-Zhu Xu,Yang Xue,Si-Qi Wei,Jia-Heng Li,Richard J. Traub,Mao-De Wang,Dong-Yuan Cao,Valproate reverses stress-induced somatic hyperalgesia and visceral hypersensitivity by up-regulating spinal 5-HT2C receptor expression in female rats,Neuropharmacology,
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:4000

Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
M00227-4 usage in article: APP:WB, SAMPLE:MSC-LIPO, DILUTION:NA

Qi Xiong,Xiang Tian,Weiling Li,Lin Chen,Mei Zhou,Congyue Xu,Qin Ru Sulforaphane alleviates methamphetamine-induced oxidative damage and apoptosis via the Nrf2-mediated pathway in vitro and in vivo.Food and Agricultural Immunology31(1)2020.https://doi.org
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:PC12 CELLS, DILUTION:1:1000

Ling Shao,Jiuru Zhao,Qian Tang, Non-muscle myosin heavy chain 9 is a critical factor for infectious pancreatic necrosis virus cellular entry, Aquaculture,2020,736138,ISSN 0044-8486,https://doi.org/10.1016/j.aqu aculture.2020.736138.
Species: Chinook salmon, infectious Pancreatic Necrosis Virus (IPNV)
M00227-4 usage in article: APP:WB, SAMPLE:CHSE-214 CELLS, DILUTION:NA

Pei-pei Fang,Chen-wei Pan,Wei Lin,Jie Li,Shan-shan Huang,Guang-yao Zhou,Wen-jun Du,Qiang Li, "ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes",Mediators of Inflammation,vol.2020,Art
Species: Human
M00227-4 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000

Hua, D.,Tang, L.,Wang, W.,Tang, S.,Yu, L.,Zhou, X.,Wang, Q.,Sun, C.,Shi, C.,Luo, W.,Jiang, Z.,Li,H.,Yu, S.,Improved Antiglioblastoma Activity and BBB Permeability by Conjugation of Paclitaxel to a Cell‐Penetrative MMP‐2‐Cleavable Peptide. Adv.Sci.2020,200
Species: Human,Mouse
M00227-4 usage in article: APP:WB, SAMPLE:U87MG CELL AND U251 CELL, DILUTION:1:2000

Xiongcheng Xu,Yanjin Lu,Ling Zhou,Mengjiao He,Jin Zhuo,Quan Zhong,Kai Luo,Jinxin Lin,Tuning osteoporotic macrophage responses to favour regeneration by Cu-bearing titanium alloy in Porphyromonas gingiva lis lipopolysaccharide-induced microenvironments, Re
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:OSTEOPOROTIC MACROPHAGES, DILUTION:NA

Jia Song,Qin He,Xinghong Guo et al. Mesenchymal Stem Cell-conditioned Medium Alleviates High Fat-induced Hyperglucagonemia via miR-181a-5p and its Target PTEN/AKT Signaling, 28 October 2020, PREPRINT(Version 1) available at Research Square [+https://
Species: Mouse,Rat
M00227-4 usage in article: APP:WB, SAMPLE: ISLETS CELLS, αTC1-6 CELLS AND INR1G9 CELLS, DILUTION:1:500

Shan S,Xie Y,Zhang C,Jia B,Li H,Li Z.Identification of polyphenol from Ziziphi spinosae semen against human colon cancer cells and colitis-associated colorectal cancer in mice.Food Funct.2020 Sep 23;11(9):8259-8272.doi:10.1039/d0fo01375g.PMID:32966479.
Species: Human,Mouse
M00227-4 usage in article: APP:WB, SAMPLE:HCT-116 CELL AND HCT-8 CELL, DILUTION:NA

Zhang B,Zhong Q,Chen X,Wu X,Sha R,Song G,Zhang C,Chen X.Neuroprotective Effects of Celastrol on Transient Global Cerebral Ischemia Rats via Regulating HMGB1/NF-κB Signaling Pathway.Front Neurosci.2020 Aug 11;14:847.doi:10.3389/fnins.2020.00847.PMID:328485
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:1000

Li M,Xie X,Chen H,Xiong Q,Tong R,Peng C,Peng F.Aconitine induces cardiotoxicity through regulation of calcium signaling pathway in zebrafish embryos and in H9c2 cells.J Appl Toxicol.2020 Jun;40(6):780-793.doi:10.1002/jat.3943.Epub 2020 Jan 23.PMID:3197543
Species: Zebrafish,Rat
M00227-4 usage in article: APP:WB, SAMPLE:H9C2 CELL, DILUTION:NA

Singh M,George AK,Eyob W,Homme RP,Stanisic D,Tyagi SC.High methionine diet in skeletal muscle remodeling: Epigenetic mechanism of homocysteine mediated growth retardation.Can J Physiol Pharmacol.2020 Aug 15.doi:10.1139/cjpp-2020-0093.Epub ahead of print.P
Species: Mouse

Yuan Y,Li B,Kuang Y,Ni S,Zhuge A,Yang J,Lv L,Gu S,Yan R,Li Y,Wang K,Yang L,Zhu X,Wu J,Bian X,Li L. The fiber metabolite butyrate reduces gp130 by targeting TRAF5 in colorectal cancer cells. Cancer Cell Int.2020 Jun 3;20:212.doi:10. 1186/s12935-020-01305-9
Species: Human,Mouse
M00227-4 usage in article: APP:WB, SAMPLE:HCT-116 CELL AND HT-29 CELL, DILUTION:1:1000

Ni S,Kuang Y,Yuan Y,Yu B. Mitochondrion-mediated iron accumulation promotes carcinogenesis and Warburg effect through reactive oxygen species in osteosarcoma. Cancer Cell Int.2020 Aug 18;20:399. doi:10.1186/s12935-020-01494-3.PMID:32831652;PMCID:PMC743701
Species: Human,Mouse
M00227-4 usage in article: APP:WB, SAMPLE:SAOS-2 CELL AND U2OS CELL, DILUTION:1:1000

Liu L,Liu F,Sun Z,Peng Z,You T,Yu Z. LncRNA NEAT1 promotes apoptosis and inflammation in LPS-induced sepsis models by targeting miR-590-3p. Exp Ther Med.2020 Oct; 20(4):3290-3300. doi:10.3892/etm.2020.9079. Epub 2020 Jul 29.PMID:32855700;PMCID:PMC7444425.
Species: Human
M00227-4 usage in article: APP:WB, SAMPLE:H9C2 CELL, DILUTION:1:2000

Fan YN,Li C,Huang L,Chen L,Tang Z,Han G,Liu Y.Characterization of Group I Metabotropic Glutamate Receptors in Rat and Human Adrenal Glands.Front Physiol.2020 May 27;11:401.doi:10.3389/fphys.2020.00401. PMID:32536873;PMCID:PMC7267184.
Species: Rat,Human
M00227-4 usage in article: APP:WB, SAMPLE:ADRENAL MEDULLA, DILUTION:1:1000

Chen H,Sheng H,Zhao Y,Zhu G.Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway.Med Sci Monit.2020 Dec 31;26:e928403.doi:10.12659/MSM.928403.PMID:33382
Species: Human,Mouse
M00227-4 usage in article: APP:WB, SAMPLE: SNU-16 CELL, DILUTION:NA

Duan Q,Huang FL,Li SJ,Chen KZ,Gong L,Qi J,Yang ZH,Yang TL,Li F,Li CQ.BET proteins inhibitor JQ-1 impaired the extinction of remote auditory fear memory: An effect mediated by insulin like growth factor 2.Neuropharmacology.2020 Oct 15;177:108255.doi:10.101
Species: Mouse
M00227-4 usage in article: APP:WB, SAMPLE:ACC AND HIPPOCAMPI, DILUTION:1:8000

Yang R,Li Y,Mehmood S,Yan C,Huang Y,Cai J,Ji J,Pan W,Zhang W,Chen Y.Polysaccharides from Armillariella tabescens mycelia ameliorate renal damage in type 2 diabetic mice.Int J Biol Macromol.2020 Nov 1;162: 1682-1691.doi:10.1016/j.ijbiomac.2020.08.006.Epub
Species: Mouse
M00227-4 usage in article: APP:WB, SAMPLE:RENAL AND COLONIC TISSUE, DILUTION:NA

Chen H,Zhu Y,Zhao X,He H,Luo J,Ao Y,Wang H.Prenatal ethanol exposure increased the susceptibility of adult offspring rats to glomerulosclerosis.Toxicol Lett.2020 Mar 15;321:44-53.doi:10.1016/j.toxlet.2019.11. 026. Epub 2019 Dec 4.PMID:31811911.
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:5000

Sun L,Hu C,Wang Z,Zhang X.MiR-182 inhibits kidney fibrosis by regulating transforming growth factor β1/Smad3 pathway in autosomal dominant polycystic kidney disease.IUBMB Life.2020 Jul;72(7):1340-1348.doi: 10.1002/iub.2255.Epub 2020 Feb 17.PMID:32067333.
Species: Mouse
M00227-4 usage in article: APP:WB, SAMPLE:KIDNEY CYST LINED EPITHELIAL CELL, DILUTION:1:3000

Li X,Zuo C,Wu M,Zhang Z.Linc-ROR promotes arsenite-transformed keratinocyte proliferation by inhibiting P53 activity.Metallomics.2020 Jun 24;12(6):963-973.doi:10.1039/d0mt00076k.PMID:32373892.
Species: Human
M00227-4 usage in article: APP:WB, SAMPLE:HACAT CELL, DILUTION:1:500

Lin C,Zhang Y,Wang J,Sui A,Xiu L,Zhu X.The study of effect and mechanism of 630-nm laser on human lung adenocarcinoma cell xenograft model in nude mice mediated by hematoporphyrin derivatives.Lasers Med Sci.2020 Jul;35(5):1085-1094.doi:10.1007/s10103-019-
Species: Mouse

Jing X,Du T,Yang X,Zhang W,Wang G,Liu X,Li T,Jiang Z.Desferoxamine protects against glucocorticoid-induced osteonecrosis of the femoral head via activating HIF-1α expression.J Cell Physiol.2020 Dec;235(12):9864-9875.doi:10.1002/jcp.29799.Epub 2020 May 21.
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:RAT BMSCS, DILUTION:NA

Lin S,Zhang G,Zhao Y,Shi D,Ye Q,Li Y,Wang S.Methylation and serum response factor mediated in the regulation of gene ARRDC3 in breast cancer.Am J Transl Res.2020 May 15;12(5):1913-1927.PMID:32509187;PMCID:PMC7270002.
Species: Human
M00227-4 usage in article: APP:WB, SAMPLE:NORMAL AND TUMOROUS BREAST TISSUE, DILUTION:NA

Zhang L,Meng X,Pan C,Qu F,Gan W,Xiang Z,Han X,Li D.piR-31470 epigenetically suppresses the expression of glutathione S-transferase pi 1 in prostate cancer via DNA methylation.Cell Signal.2020 Mar;67:10950 1.doi:10.1016/j.cellsig.2019.109501.Epub 2019 Dec
Species: Human
M00227-4 usage in article: APP:WB, SAMPLE:PC-3 CELLS AND DU145 CELLS, DILUTION:NA

Yang R,Jia Q,Mehmood S,Ma S,Liu X.Genistein ameliorates inflammation and insulin resistance through mediation of gut microbiota composition in type 2 diabetic mice.Eur J Nutr.2020 Oct 17.doi:10.1007/s0 0394-020-02403-0.Epub ahead of print.PMID:33068158.
Species: Mouse
M00227-4 usage in article: APP:WB, SAMPLE:COLON TISSUE, DILUTION:NA

Liao H,Li Y,Zhang X,Zhao X,Zheng D,Shen D,Li R. Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation.J Immunol Res.2020 Aug 27;2020:8263598.doi:10.1155/2020/ 8263598.PMID
Species: Mouse
M00227-4 usage in article: APP:WB, SAMPLE:MACROPHAGES, DILUTION:1:1000

Sun K,Luo J,Jing X,Xiang W,Guo J,Yao X,Liang S,Guo F,Xu T.Hyperoside ameliorates the progression of osteoarthritis: An in vitro and in vivo study.Phytomedicine.2020 Oct 14;80:153387.doi:10.1016/j.phymed.2020. 153387.Epub ahead of print.PMID:33130473.
Species: Mouse
M00227-4 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION:1:300

Wei JL,Wu CJ,Chen JJ,Shang FT,Guo SG,Zhang XC,Liu H.LncRNA NEAT1 promotes the progression of sepsis-induced myocardial cell injury by sponging miR-144-3p.Eur Rev Med Pharmacol Sci.2020 Jan;24(2):851-861.doi:10.26355/eurrev_202001_20069.PMID:32016991.
Species: Mouse
M00227-4 usage in article: APP:WB, SAMPLE:HL-1 CELLS, DILUTION:1:2000

Tan X,Zeng Y,Tu Z,Li P,Chen H,Cheng L,Tu S,Jiang L.TRPV1 Contributes to the Neuroprotective Effect of Dexmedetomidine in Pilocarpine-Induced Status Epilepticus Juvenile Rats.Biomed Res Int.2020 Apr 7;2020:7623635.doi:10.1155/2020/7623635.PMID:32337274;PMC
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:10000

Li Y,Feng Z,Xing S,Liu W,Zhang G.Combination of serum matrix metalloproteinase-3 activity and EBV antibodies improves the diagnostic performance of nasopharyngeal carcinoma.J Cancer.2020 Aug 18;11(20):6009-6018.doi:10.7150/jca.46977.PMID:32922541; PMCID:P
Species: Human
M00227-4 usage in article: APP:WB, SAMPLE:CNE1 CELLS,C666-1 CELLS AND MRC5 CELLS, DILUTION:1:5000

Shan W,Han F,Xu Y,Shi Y.Stathmin Regulates Spatiotemporal Variation in the Memory Loop in Single-Prolonged Stress Rats. J Mol Neurosci. 2020 Apr;70(4):576-589.doi: 10.1007/s12031-019-01459-w.Epub 2020 Jan 13.PMID: 31933182.
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:4000

Ma L,Chen X,Zhao B,Shi Y,Han F.Enhanced apoptosis and decreased ampa receptors are involved in deficit in fear memory in rin1 knockout rats.J Affect Disord.2020 May 1;268:173-182.doi:10.1016/j.jad.2020.02.040. Epub 2020 Feb 27.PMID:32174475.
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS TISSUE, DILUTION:1:500

Zhang J,Huang L,Shi X,Yang L,Hua F,Ma J,Zhu W,Liu X,Xuan R,Shen Y,Liu J,Lai X,Yu P. Metformin protects against myocardial ischemia-reperfusion injury and cell pyroptosis via AMPK/NLRP3 inflammasome pathway. Aging (Albany NY).2020 Nov 24;12.doi:10.18632/ag
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:CARDIOMYOCYTES, DILUTION:1:2000

Qin L,Zang M,Xu Y,Zhao R,Wang Y,Mi Y,Mei Y.Chlorogenic Acid Alleviates Hyperglycemia-Induced Cardiac Fibrosis through Activation of the NO/cGMP/PKG Pathway in Cardiac Fibroblasts.Mol Nutr Food Res.2020 Nov 17:e2000810.doi:10.1002/mnfr.202000810.Epub ahead
Species: Mouse
M00227-4 usage in article: APP:WB, SAMPLE:VENTRICLES, DILUTION:NA

Xu Q,Liu M,Zhang F,Liu X,Ling S,Chen X,Gu J,Ou W,Liu S,Liu N. Ubiquitin-specific protease 2 regulates Ang Ⅱ-induced cardiac fibroblasts activation by up-regulating cyclin D1 and stabilizing β-catenin in vitro. J Cell Mol Med.2020 Dec 12.doi:10.1111/jcmm.1
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:CFS, DILUTION:NA

Yuan WM,Fan YG,Cui M,Luo T,Wang YE,Shu ZJ,Zhao J,Zheng J,Zeng Y. SOX5 Regulates Cell Proliferation, Apoptosis, Migration and Invasion in KSHV-Infected Cells. Virol Sin.2020 Nov 24.doi:10.1007/s12250-020-00313-3.Epub ahead of print.PMID:33231856.
Species: Human
M00227-4 usage in article: APP:WB, SAMPLE:CKS TISSUES AND BAC CELLS, DILUTION:1:500

Wang P,Wang C,Liu C. Antitumor effects of dioscin in A431 cells via adjusting ATM/p53-mediated cell apoptosis, DNA damage and migration. Oncol Lett. 2021 Jan;21(1):59.doi:10.3892/ol.2020.12321.Epub 2020 Nov 19.PMID:33281970;PMCID:PMC7709553.
Species: Human
M00227-4 usage in article: APP:WB, SAMPLE:A431 CELLS, DILUTION:NA

He L,Wang Z,Zhou R,Xiong W,Yang Y,Song N,Qian J. Dexmedetomidine exerts cardioprotective effect through miR-146a-3p targeting IRAK1 and TRAF6 via inhibition of the NF-κB pathway. Biomed Pharmacother.2021 Jan;133:110993.doi:10.1016/j.biopha.2020.110993.Epu
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:H9C2 CELL, DILUTION:1:1000

MiR%u201030a%u20105p ameliorates spinal cord injury%u2010induced inflammatory responses and oxidative stress by targeting Neurod 1 through MAPK/ERK signalling

Hypoxia inducible factor 1%u03B1 promotes survival of mesenchymal stem cells under hypoxia

Astragaloside IV inhibits ventricular remodeling and improves fatty acid utilization in rats with chronic heart failure

The relationship between bile acid concentration, glucagon-like-peptide 1, fibroblast growth factor 15 and bile acid receptors in rats during progression of %u2026

Puerarin protects rat brain against ischemia/reperfusion injury by suppressing autophagy via the AMPK-mTOR-ULK1 signaling pathway

Research on Roles of Mongolian Medical Warm Acupuncture in Inhibiting p38 MAPK Activation and Apoptosis of Nucleus Pulposus Cells

Glucagon-like peptide-1 receptor agonists inhibit hepatic stellate cell activation by blocking the p38 MAPK signaling pathway

Orally administered berberine modulates hepatic lipid metabolism by altering microbial bile acid metabolism and the intestinal FXR signaling pathway

Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter

Alterations in necroptosis during ALDH2%u2011mediated protection against high glucose%u2011induced H9c2 cardiac cell injury

WNT10A/%u03B2%u2011catenin pathway in tumorigenesis of papillary thyroid carcinoma

microRNA-130a is an oncomir suppressing the expression of CRMP4 in gastric cancer

Age-dependent down-regulation of hyperpolarization-activated cyclic nucleotide-gated channel 4 causes deterioration of canine sinoatrial node function

The legacy effects of electromagnetic fields on bone marrow mesenchymal stem cell self-renewal and multiple differentiation potential

Polydatin attenuates diet-induced nonalcoholic steatohepatitis and fibrosis in mice

CXCL8 Antagonist Improves Diabetic Nephropathy in Male Mice With Diabetes and Attenuates High Glucose%u2013Induced Mesangial Injury

KPNA2 promotes metabolic reprogramming in glioblastomas by regulation of c-myc

Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC)

The putative tumour suppressor miR-1-3p modulates prostate cancer cell aggressiveness by repressing E2F5 and PFTK1

Dicer regulates non-homologous end joining and is associated with chemosensitivity in colon cancer patients

Expression of Hsp70 reveals significant differences between fin regeneration and inflammation in Paramisgurnus dabryanus

Rs12976445 Polymorphism Is Associated with Post-Ablation Recurrence of Atrial Fibrillation by Modulating the Expression of MicroRNA-125a and Interleukin %u2026

High levels of glucose promote the activation of hepatic stellate cells via the p38-mitogen-activated protein kinase signal pathway

Synergistic effect of TRAIL and irradiation in elimination of glioblastoma stem-like cells

The notch pathway promotes NF-%u03BAB activation through Asb2 in T cell acute lymphoblastic leukemia cells

HDAC6 inhibition suppresses chondrosarcoma by restoring the expression of primary cilia

Radiation-induced G2/M arrest rarely occurred in glioblastoma stem-like cells

Wear particles enhance autophagy through up-regulation of CD147 to promote osteoclastogenesis

Phenylephrine promotes cardiac fibroblast proliferation through calcineurin-NFAT pathway

Kaempferol inhibits interleukin%u20111%u03B2 stimulated matrix metalloproteinases by suppressing the MAPK%u2011associated ERK and P38 signaling pathways

DYT-40, a novel synthetic 2-styryl-5-nitroimidazole derivative, blocks malignant glioblastoma growth and invasion by inhibiting AEG-1 and NF-?B signaling pathways

Inhibition of STAT1 sensitizes radioresistant nasopharyngeal carcinoma cell line CNE-2R to radiotherapy

NDRG4 promotes myogenesis via Akt/CREB activation

Alleviation of Toxicity Caused by Overactivation of Ppar? through Ppar?-Inducible miR-181a2

The role of copper transporter ATP7A in platinum-resistance of esophageal squamous cell cancer (ESCC)

Combined effects of interleukin-1? and cyclic stretching on metalloproteinase expression in corneal fibroblasts in vitro

Regulation of tumorigenesis in oral epithelial cells by defined reprogramming factors Oct4 and Sox2

miR-506 inhibits cell proliferation and invasion by targeting TET family in colorectal cancer

Knockdown of versicanV1 induces a severe inflammatory response in LPS-induced acute lung injury via the TLR2-NF-?B signaling pathway in C57BL/6J mice

Iron-Mediated Lysosomal Membrane Permeabilization in Ethanol-Induced Hepatic Oxidative Damage and Apoptosis: Protective Effects of Quercetin

Targeting the D1-N-methyl-D-aspartate receptor complex reduces L-dopa-induced dyskinesia in 6-hydroxydopamine-lesioned Parkinson's rats

SIRT1 protects against myocardial ischemia?reperfusion injury via activating eNOS in diabetic rats

Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD

Dasatinib suppresses invasion and induces apoptosis in nasopharyngeal carcinoma

TSPAN8 promotes gastric cancer growth and metastasis via ERK MAPK pathway

Clinicopathologic and prognostic significance of p21 (Cip1/Waf1) expression in bladder cancer.

EGFR tyrosine kinase inhibitors promote pro-caspase-8 dimerization that sensitizes cancer cells to DNA-damaging therapy

High-density lipoprotein induces cyclooxygenase-2 expression and prostaglandin I-2 release in endothelial cells through sphingosine kinase-2

mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27

Cytochrome P450 26A1 modulates natural killer cells in mouse early pregnancy

Microarray-based identification of genes associated with cancer progression and prognosis in hepatocellular carcinoma

Inhibition of SDF-1?/CXCR4 Signalling in Subchondral Bone Attenuates Post-Traumatic Osteoarthritis

Glucagon-like peptide-1 protects cardiomyocytes from advanced oxidation protein product-induced apoptosis via the PI3K/Akt/Bad signaling pathway.

Quercetin Alleviates High-Fat Diet-Induced Oxidized Low-Density Lipoprotein Accumulation in the Liver: Implication for Autophagy Regulation

miR-124 and miR-506 inhibit colorectal cancer progression by targeting DNMT3B and DNMT1

Knockdown of Gli1 by small-interfering RNA enhances the effects of BCNU on the proliferation and apoptosis of glioma U251 cells

Inhibition of DNA methyltransferase as a novel therapeutic strategy to overcome acquired resistance to dual PI3K/mTOR inhibitors

Cai XS, Tan ZG, Li JJ, Gao WH, Li SJ, Li JL, Tang YM, Li HW, Hui HX. Med Sci Monit. 2017 Sep 8;23:4334-4342. Glucagon-Like Peptide-1 (GLP-1) Treatment Ameliorates Cognitive Impairment by Attenuating Arc Expression in Type 2 Diabetic Rats

Li X, Wang MH, Qin C, Fan WH, Tian DS, Liu JL. PLoS One. 2017 Nov 29;12(11):e0188748. doi: 10.1371/journal.pone.0188748. eCollection 2017. Fingolimod suppresses neuronal autophagy through the mTOR/p70S6K pathway and alleviates ischemic brain damag...

Jiang Q, Ma L, Li R, Sun J. Oncotarget. 2017 Dec 1;9(15):11989-11998. doi: 10.18632/oncotarget.22857. eCollection 2018 Feb 23. Colon cancer-induced interleukin-35 inhibits beta-catenin-mediated pro-oncogenic activity

Hu Y, Song J, Liu L, Zhang Y, Wang L, Li Q. Front Cell Infect Microbiol. 2018 Apr 9;8:110. doi: 10.3389/fcimb.2018.00110. eCollection 2018. microRNA-4516 Contributes to Different Functions of Epithelial Permeability Barrier by Targeting Poliovirus...

Wang W, Qing X, Wang B, Ma K, Wei Y, Shao Z. Evid Based Complement Alternat Med. 2018 Mar 12;2018:6719460. doi: 10.1155/2018/6719460. eCollection 2018. Tauroursodeoxycholic Acid Protects Nucleus Pulposus Cells from Compression-Induced Apoptosis an...

Feng X, Lin J, Xing S, Liu W, Zhang G. BMC Cancer. 2017 Jan 31;17(1):90. doi: 10.1186/s12885-017-3068-0. Higher IGFBP-1 to IGF-1 serum ratio predicts unfavourable survival in patients with nasopharyngeal carcinoma

Liao Y, Xing S, Xu B, Liu W, Zhang G. Oncotarget. 2017 May 2;8(18):30050-30062. doi: 10.18632/oncotarget.16274. Evaluation of the circulating level of fibroblast activation protein α for diagnosis of esophageal squamous cell carcinoma

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Lu Y, Zhang K, Li C, Yao Y, Tao D, Liu Y, Zhang S, Ma Y. Plos One. 2012;7(1):E30999. Doi: 10.1371/Journal.Pone.0030999. Epub 2012 Jan 27. Piwil2 Suppresses P53 By Inducing Phosphorylation Of Signal Transducer And Activator Of Transcription 3 In Tu...

Meng R, Zhu D, Bi Y, Yang D, Wang Y. Plos One. 2013;8(1):E53557. Doi: 10.1371/Journal.Pone.0053557. Epub 2013 Jan 10. Erythropoietin Inhibits Gluconeogenesis And Inflammation In The Liver And Improves Glucose Intolerance In High-Fat Diet-Fed Mice.

Chen C, Mei H, Shi W, Deng J, Zhang B, Guo T, Wang H, Hu Y. Plos One. 2013 Apr 10;8(4):E60860. Doi: 10.1371/Journal.Pone.0060860. Print 2013. Egfp-Egf1-Conjugated Plga Nanoparticles For Targeted Delivery Of Sirna Into Injured Brain Microvascular E...

Zafar Mi, Hu C, Liu D, Shafqat Ra, Gao F. J Diabetes Res. 2014;2014:458104. Doi: 10.1155/2014/458104. Epub 2014 Aug 14. Insulin Detemir Causes Lesser Weight Gain In Comparison To Insulin Glargine: Role On Hypothalamic Npy And Galanin.

Zhao Y, Xie P, Fan H. Environ Sci Technol. 2012 Jan 3;46(1):34-41. Doi: 10.1021/Es201514H. Epub 2011 Sep 19. Genomic Profiling Of Micrornas And Proteomics Reveals An Early Molecular Alteration Associated With Tumorigenesis Induced By Mc-Lr In Mice.

Zhu W, Chen S, Li Z, Zhao X, Li W, Sun Y, Zhang Z, Ling W, Feng X. Nutr Metab (Lond). 2014 Aug 12;11:35. Doi: 10.1186/1743-7075-11-35. Ecollection 2014. Effects And Mechanisms Of Resveratrol On The Amelioration Of Oxidative Stress And Hepatic Stea...

Zhai B, Liu H, Li X, Dai L, Gao Y, Li C, Zhang L, Ding Y, Yu X, Zhang J. Cell Physiol Biochem. 2013;32(2):264-78. Doi: 10.1159/000354435. Epub 2013 Jul 31. Bmp15 Prevents Cumulus Cell Apoptosis Through Ccl2 And Fbn1 In Porcine Ovaries.

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16 Customer Q&As for Anti-GAPDH Antibody

Question

My question regarding product M00227-4, anti-GAPDH antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

C. Rodriguez

Verified customer

Asked: 2020-04-17

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00227-4 anti-GAPDH antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-17

Question

I see that the anti-GAPDH antibody M00227-4 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-02-20

Question

Our lab want to know about using your anti-GAPDH antibody for gluconeogenesis studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-12-09

Answer

Thank you for your inquiry. This M00227-4 anti-GAPDH antibody is validated on hela cells. It is guaranteed to work for IF, IHC, WB in bovine, chicken, equine, human, mouse, pig, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-12-09

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for muscle using anti-GAPDH antibody M00227-4. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-21

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-21

Question

I was wanting to use your anti-GAPDH antibody for WB for pig muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for pig muscle identification?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

You can see on the product datasheet, M00227-4 anti-GAPDH antibody has been tested for IF, IHC, WB on bovine, chicken, equine, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in pig muscle in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-05

Question

Here is the WB image, lot number and protocol we used for muscle using anti-GAPDH antibody M00227-4. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-11-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-27

Question

We are currently using anti-GAPDH antibody M00227-4 for chicken tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says bovine, chicken, equine, human, mouse, pig, rat. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2018-08-27

Answer

The anti-GAPDH antibody (M00227-4) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-08-27

Question

Do you have a BSA free version of anti-GAPDH antibody M00227-4 available?

Verified Customer

Verified customer

Asked: 2018-07-20

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GAPDH antibody M00227-4 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-07-20

Question

Does anti-GAPDH antibody M00227-4 work for WB with muscle?

G. Bhatt

Verified customer

Asked: 2018-01-16

Answer

According to the expression profile of muscle, GAPDH is highly expressed in muscle. So, it is likely that anti-GAPDH antibody M00227-4 will work for WB with muscle.

Boster Scientific Support

Answered: 2018-01-16

Question

Is this M00227-4 anti-GAPDH antibody reactive to the isotypes of GAPDH?

Verified Customer

Verified customer

Asked: 2017-11-06

Answer

The immunogen of M00227-4 anti-GAPDH antibody is Native porcine purified protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-11-06

Question

Our team were content with the WB result of your anti-GAPDH antibody. However we have observed positive staining in lung cytosol using this antibody. Is that expected? Could you tell me where is GAPDH supposed to be expressed?

H. Taylor

Verified customer

Asked: 2017-10-02

Answer

From what I have seen in literature, lung does express GAPDH. Generally GAPDH expresses in cytoplasm, cytosol. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305

Boster Scientific Support

Answered: 2017-10-02

Question

We bought anti-GAPDH antibody for IF on lung in a previous project. I am using bovine, and I plan to use the antibody for WB next. We want examining lung as well as colon adenocarcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2017-09-20

Answer

I looked at the website and datasheets of our anti-GAPDH antibody and it seems that M00227-4 has been tested on bovine in both IF and WB. Thus M00227-4 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in bovine even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-09-20

Question

We have been able to see staining in mouse colon adenocarcinoma. Do you have any suggestions? Is anti-GAPDH antibody supposed to stain colon adenocarcinoma positively?

R. Johnson

Verified customer

Asked: 2016-11-07

Answer

From literature colon adenocarcinoma does express GAPDH. From Uniprot.org, GAPDH is expressed in smooth muscle tissue, liver, lung, placenta, astrocytoma, leukemia, colon adenocarcinoma, eye, kidney, lung, lymph placenta, muscle, platelet, prostatic carcinoma, brain, cajal-retzius cell fetal brain cortex, heart, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305

Boster Scientific Support

Answered: 2016-11-07

Question

Will M00227-4 anti-GAPDH antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

M. Banerjee

Verified customer

Asked: 2015-07-17

Answer

As indicated on the product datasheet, M00227-4 anti-GAPDH antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-07-17

Question

Is a blocking peptide available for product anti-GAPDH antibody (M00227-4)?

L. Williams

Verified customer

Asked: 2014-12-24

Answer

We do provide the blocking peptide for product anti-GAPDH antibody (M00227-4). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2014-12-24

Question

I am interested in to test anti-GAPDH antibody M00227-4 on pig muscle for research purposes, then I may be interested in using anti-GAPDH antibody M00227-4 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

V. Jackson

Verified customer

Asked: 2014-02-12

Answer

The products we sell, including anti-GAPDH antibody M00227-4, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-02-12

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M00227-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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