Anti-Gli1 Rabbit Monoclonal Antibody
|Product Name||Anti-Gli1 Rabbit Monoclonal Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.|
|Description||Anti-Gli1 Rabbit Monoclonal Antibody tested for WB in Human|
|Cite This Product||Anti-Gli1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00527)|
|Contents/Buffer||Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|Immunogen||A synthesized peptide derived from human Gli1|
Assay Dilutions Overview
Images And Assay Conditions
Western blot analysis of Gli1 expression in A549 cell lysate (M00527).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLI1 monoclonal antibody (Catalog # M00527) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLI1
Protein Target Info (Source: Uniprot.org)
|Protein Name||Zinc finger protein GLI1|
|Tissue Specificity||Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. .|
|Alternative Names||Zinc finger protein GLI1;Glioma-associated oncogene;Oncogene GLI;GLI1;GLI;|
|Subcellular Localization||Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.|
|Molecular Weight||117904 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. .|
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