Anti-Gli1 Rabbit Monoclonal Antibody


SKU M00527
Size 100ug/vial
Reactivity Human
Clonality Monoclonal
Clone Number GCG-7
Host Rabbit
Applications WB

Overview

Product Name Anti-Gli1 Rabbit Monoclonal Antibody
SKU/Catalog Number M00527
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-Gli1 Rabbit Monoclonal Antibody tested for WB in Human
Cite This Product Anti-Gli1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00527)
Host Rabbit
Contents/Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Immunogen A synthesized peptide derived from human Gli1
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB 1:500-1:1000

Images And Assay Conditions

Western blot analysis of Gli1 expression in A549 cell lysate (M00527).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLI1 monoclonal antibody (Catalog # M00527) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLI1

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P08151
Gene Name GLI1
Protein Name Zinc finger protein GLI1
Tissue Specificity Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. .
Alternative Names Zinc finger protein GLI1;Glioma-associated oncogene;Oncogene GLI;GLI1;GLI;
Subcellular Localization Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.
Molecular Weight 117904 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. .
Research Areas Human

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Monoclonal antibody for GLI-1/GLI1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB. Reactive species: Human GLI-1/GLI1 information: Molecular Weight: 117904 MW; Subcellular Localization: Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1; Tissue Specificity: Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract.
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In stock
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M00527
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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Publications

Duan F, Lin M, Li C, Ding X, Qian G, Zhang H, Ge S, Fan X, Li J. Cancer Biol Ther. 2014 May;15(5):544-59. Doi: 10.4161/Cbt.28157. Epub 2014 Mar 11. Effects Of Inhibition Of Hedgehog Signaling On Cell Growth And Migration Of Uveal Melanoma Cells.
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