Product Info Summary
SKU: | A01272-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Glutaminase GLS Antibody
View all Glutaminase Antibodies
SKU/Catalog Number
A01272-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Glutaminase GLS Antibody catalog # A01272-1. Tested in WB,ICC/IF,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glutaminase GLS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01272-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human ACBP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01272-1 is reactive to GLS in Human, Mouse, Rat
Applications
A01272-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
73.461kDa
Background of Glutaminase
Key calcium ion sensor involved in the Ca2+-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress By similarity.
Delia J. Hern¨¢ndez-Deviez, Hum. Mol. Genet., Jan 2006; 15: 129 - 142.
Yanchao Huang, FASEB J, Mar 2007; 21: 732 - 742.
Mengfatt Ho, Hum. Mol. Genet., Sep 2004; 13: 1999 - 2010.
Eriko Fujita, Hum. Mol. Genet., Mar 2007; 16: 618 - 629.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of GLS using Anti-Glutaminase GLS Antibody (A01272-1).
ICC staining Glutaminase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Glutaminase GLS Antibody A01272-1
Western blot analysis of Glutaminase on different lysates using anti-Glutaminase antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For GLS (Source: Uniprot.org, NCBI)
Gene Name
GLS
Full Name
Glutaminase kidney isoform, mitochondrial
Weight
73.461kDa
Superfamily
glutaminase family
Alternative Names
AAD20; EC 3.5.1.2; GLS; GLS1DKFZp686O15119; glutaminase C; glutaminase kidney isoform, mitochondrial; Glutaminase; glutaminase, phosphate-activated; K-Glutaminase; KIAA0838FLJ10358; L-Glutamine Amidohydrolase GLS AAD20, CASGID, DEE71, EIEE71, GAC, GAM, GDPAG1, KGA, GLS glutaminase glutaminase kidney isoform, mitochondrial|K-glutaminase|L-glutamine amidohydrolase|glutaminase C|glutaminase, phosphate-activated
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GLS, check out the GLS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GLS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glutaminase GLS Antibody (A01272-1)
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No publications found for A01272-1
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Customer Reviews
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