Product Info Summary
SKU: | A01019-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Glutathione peroxidase 1 GPX1 Antibody
View all Glutathione Peroxidase 1/GPX1 Antibodies
SKU/Catalog Number
A01019-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Glutathione peroxidase 1 GPX1 Antibody catalog # A01019-1. Tested in WB,ICC/IF,IHC,IP applications. This antibody reacts with Human,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glutathione peroxidase 1 GPX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01019-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant protein of human SLC7A5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01019-1 is reactive to GPX1 in Human, Rat
Applications
A01019-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
22.088kDa
Background of Glutathione Peroxidase 1/GPX1
4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Gingras AC, et al. (1998) Genes Dev 12(4): 502-513.
Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904.
Kumar V, et al. (2000) EMBO J 19(5): 1087-1097.
Moody CA, et al. (2005) J Virol 79(9): 5499-5506.
Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of GPX1 using Anti-Glutathione peroxidase 1 GPX1 Antibody (A01019-1).
ICC staining Glutathione Peroxidase 1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Glutathione peroxidase 1 GPX1 Antibody A01019-1
Western blot analysis of Glutathione Peroxidase 1 on THP-1 cells lysates using anti-Glutathione Peroxidase 1 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For GPX1 (Source: Uniprot.org, NCBI)
Gene Name
GPX1
Full Name
Glutathione peroxidase 1
Weight
22.088kDa
Superfamily
glutathione peroxidase family
Alternative Names
Cellular glutathione peroxidase; CGPx; EC 1.11.1; EC 1.11.1.9; Glutathione Peroxidase 1; GPX1; GPx-1; GSHPX1; GSHPx-1; MGC14399; MGC88245 GPX1 GPXD, GSHPX1 glutathione peroxidase 1 glutathione peroxidase 1|GSHPx-1|cellular glutathione peroxidase|selenoprotein GPX1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GPX1, check out the GPX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glutathione peroxidase 1 GPX1 Antibody (A01019-1)
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No publications found for A01019-1
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