Click image to see more details
Product Info Summary
| SKU: | RP1062 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Hepatocyte growth factor HGF Antibody
SKU/Catalog Number
RP1062
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Hepatocyte growth factor HGF Antibody catalog # RP1062. Tested in WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hepatocyte growth factor HGF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1062)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse HGF, different from the related human sequence by five amino acids, and from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1062 is reactive to HGF in Mouse
Applications
RP1062 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
34 kDa
Calculated molecular weight
83.134kDa
Background of HGF
Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenic factor. This gene is mapped to 7q21.11. It is secreted by mesenchymal cells and targets and acts primarily uponepithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells. It has been shown to have a major role in embryonic organ development, specifically in myogenesis, in adult organ regeneration and in wound healing. HGF can regulate cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. HGF also serves as a paracrine mediator to control placental development and growth. HGF was identified as one of the liver sinusoidal endothelial cell-derived paracrine mediators promoting hepatocyte growth.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-HGF antibody, RP1062, Western blotting
All lanes: Anti HGF (RP1062) at 0.5ug/ml
WB: NIH Whole Cell Lysate at 40ug
Predicted bind size: 34KD
Observed bind size: 34KD
Protein Target Info & Infographic
Gene/Protein Information For HGF (Source: Uniprot.org, NCBI)
Gene Name
HGF
Full Name
Hepatocyte growth factor
Weight
83.134kDa
Superfamily
peptidase S1 family
Alternative Names
deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A HGF DFNB39, F-TCFB, HPTA, SF, HGF hepatocyte growth factor hepatocyte growth factor|fibroblast-derived tumor cytotoxic factor|hepatocyte growth factor (hepapoietin A; scatter factor)|hepatopoietin-A|lung fibroblast-derived mitogen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HGF, check out the HGF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HGF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hepatocyte growth factor HGF Antibody (RP1062)
Hello CJ!
RP1062 has been cited in 12 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
MicroRNA-210 promotes angiogenesis in acute myocardial infarction
Corticosterone Mediates the Inhibitory Effect of Restraint Stress on the Migration of Mesenchymal Stem Cell to Carbon Tetrachloride-Induced Fibrotic Liver by Downregulating CXCR4/7 Expression
Pro-osteogenic effects of fibrin glue in treatment of avascular necrosis of the femoral headin vivoby hepatocyte growth factor-transgenic mesenchymal stem cells
HGF-Transgenic MSCs Can Improve the Effects of Tissue Self-Repair in a Rabbit Model of Traumatic Osteonecrosis of the Femoral Head
Therapeutic role of human hepatocyte growth factor (HGF) in treating hair loss
Wen Q, Zhou L, Zhou C, Zhou M, Luo W, Ma L. J Cell Mol Med. 2012 Jun;16(6):1260-73. Doi: 10.1111/J.1582-4934.2011.01407.X. Change In Hepatocyte Growth Factor Concentration Promote Mesenchymal Stem Cell-Mediated Osteogenic Regeneration.
Yu X, He X, Jiang J, He J, Fan R, Wang H, Geng J, Dong C. Acta Histochem. 2015 Sep;117(7):624-8. Doi: 10.1016/J.Acthis.2015.06.002. Epub 2015 Jun 19. Expression And Tissue Distribution Of Hepatocyte Growth Factor (Hgf) And Its Receptor (C-Met) In ...
Hu Zp, Fang Xl, Qian Hy, Fang N, Wang Bn, Wang Y. Fundam Clin Pharmacol. 2014 Oct;28(5):501-11. Doi: 10.1111/Fcp.12057. Epub 2013 Nov 29. Telmisartan Prevents Angiotensin Ii-Induced Endothelial Dysfunction In Rabbit Aorta Via Activating Hgf/Met Sy...
Lin Y, Xie Wf, Chen Yx, Zhang X, Zeng X, Qiang H, Chen Wz, Yang Xj, Han Zg, Zhang Zb. Liver Int. 2005 Aug;25(4):796-807. Treatment Of Experimental Hepatic Fibrosis By Combinational Delivery Of Urokinase-Type Plasminogen Activator And Hepatocyte Gr...
Ding W, Sun S, Zhen J, Yu Y; Wang. J Int Med Res. 2010;38(6):1933-41. Urinary Excretion And Renal Production Of Hepatocyte Growth Factor In Children With Henoch-Sch??nlein Purpura.
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Hepatocyte growth factor HGF Antibody
Question
My question regarding product RP1062, anti-HGF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1062 anti-HGF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-25
Question
Would anti-HGF antibody RP1062 work for WB with leukocyte?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
According to the expression profile of leukocyte, HGF is highly expressed in leukocyte. So, it is likely that anti-HGF antibody RP1062 will work for WB with leukocyte.
Boster Scientific Support
Answered: 2020-03-19
Question
Is this RP1062 anti-HGF antibody reactive to the isotypes of HGF?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
The immunogen of RP1062 anti-HGF antibody is A synthetic peptide corresponding to a sequence at the N-terminus of mouse HGF (33-64aa QKKRRNTLHEFKKSAKTTLTKEDPLLKIKTKK), different from the related human sequence by five amino acids, and from the related rat sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-04
Question
Will RP1062 anti-HGF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
You can see on the product datasheet, RP1062 anti-HGF antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-13
Question
I was wanting to use your anti-HGF antibody for WB for mouse leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse leukocyte identification?
P. Edwards
Verified customer
Asked: 2019-12-27
Answer
It shows on the product datasheet, RP1062 anti-HGF antibody has been validated for WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-27
Question
We are currently using anti-HGF antibody RP1062 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
The anti-HGF antibody (RP1062) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-26
Question
I see that the anti-HGF antibody RP1062 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-02-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-13
Question
We have been able to see staining in mouse embryonic fibroblast. Are there any suggestions? Is anti-HGF antibody supposed to stain embryonic fibroblast positively?
Verified Customer
Verified customer
Asked: 2018-07-09
Answer
From literature embryonic fibroblast does express HGF. From Uniprot.org, HGF is expressed in placenta, liver, leukocyte, lung fibroblast, embryonic fibroblast, brain, among other tissues. Regarding which tissues have HGF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Embryonic fibroblast, Pubmed ID: 1831266
Leukocyte, Pubmed ID: 2145836
Liver, Pubmed ID: 2531289
Lung fibroblast, Pubmed ID: 1824873
Placenta, Pubmed ID: 1826653, 2528952
Boster Scientific Support
Answered: 2018-07-09
Question
Is a blocking peptide available for product anti-HGF antibody (RP1062)?
Verified Customer
Verified customer
Asked: 2018-06-13
Answer
We do provide the blocking peptide for product anti-HGF antibody (RP1062). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-13
Question
Our lab want to know about using your anti-HGF antibody for r-mmu-5673001; raf/map kinase cascade studies. Has this antibody been tested with western blotting on nih whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-02-27
Answer
Thanks for your inquiry. This RP1062 anti-HGF antibody is validated on nih whole cell lysate. It is guaranteed to work for WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-02-27
Question
Do you have a BSA free version of anti-HGF antibody RP1062 available?
F. Zhang
Verified customer
Asked: 2017-09-13
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HGF antibody RP1062 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-13
Question
you antibody to test anti-HGF antibody RP1062 on mouse leukocyte for research purposes, then I may be interested in using anti-HGF antibody RP1062 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-07-07
Answer
The products we sell, including anti-HGF antibody RP1062, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-HGF antibody RP1062. Let me know if you need anything else.
Z. Li
Verified customer
Asked: 2017-04-10
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-04-10
Question
Here is the WB image, lot number and protocol we used for leukocyte using anti-HGF antibody RP1062. Please let me know if you require anything else.
A. Roberts
Verified customer
Asked: 2013-06-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-06-07
