Anti-Histone H1 HIST1H1B Antibody

Product Name

Anti-Histone H1 HIST1H1B Antibody

View all H1-5 Antibodies

SKU/Catalog Number

A06717

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Histone H1 HIST1H1B Antibody catalog # A06717. Tested in EILSA, IF, IHC-P, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Histone H1 HIST1H1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06717)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

2B4

Isotype

IgG

Immunogen

Synthesized peptide derived from human Histone H1 around the non-phosphorylation site of T17.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06717 is reactive to H1-5 in Human, Mouse

Applications

A06717 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

22.58kDa

Background of H1-5

Nuclear transcription factor Y subunit beta is a component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000

Validation Images & Assay Conditions

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Western blot analysis of 3T3 cells using Histone H1 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

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Western blot analysis of various cells using Histone H1 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Gene/Protein Information For H1-5 (Source: Uniprot.org, NCBI)

Alternative Names

Histone H1.5 H1-5 H1, H1.5, H1B, H1F5, H1s-3, HIST1H1B H1.5 linker histone, cluster member histone H1.5|H1 histone family, member 5|histone 1, H1b|histone H1a|histone H1b|histone H1s-3|histone cluster 1 H1 family member b|histone cluster 1, H1b

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