Anti-Histone H4 HIST1H4A Antibody

Product Name

Anti-Histone H4 HIST1H4A Antibody

View all H4C1 Antibodies

SKU/Catalog Number

A14495

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Histone H4 HIST1H4A Antibody (Catalog # A14495). Tested in Dot blot, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Histone H4 HIST1H4A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14495)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-Histone H4 pan Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing a sequence from the N-terminal region of histone H4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A14495 is reactive to H4C1 in Human

Applications

A14495 is guaranteed for Dot blot, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

11.367kDa

Background of H4C1

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a internal role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes. Anti-Histone H4 pan Antibody is ideal for research in Chromatin Remodeling, Gene Expression and Epigenetics.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20000
WB: 1:500-1:2000
Dot Blot: 1:1000

Validation Images & Assay Conditions

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Western blot analysis of HeLa Whole Cell Lysates (lane 1), HeLa Nuclear Extract (lane 2), HeLa Whole Cell Lysates (lane 3) and HeLa Nuclear Extract (lane 4). Primary antibody: Lanes 1-2: Histone H3 K18Ac, Lanes 3-4: rabbit anti-Histone H4 polyclonal antibody (Catalog # A14495) at 1:500. Predicted/Observed size: ~15.4kDa for Histone H3, and 11.3kDa for Histone H4.

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Dot Blot of rabbit anti-Histone H4 polyclonal antibody (Catalog # A14495) at 1:1000. Lane 1: K5Ac. Lane 2: NM. Load: 100, 10, 1 picomoles of peptide.

Gene/Protein Information For H4C1 (Source: Uniprot.org, NCBI)

Alternative Names

Histone H4 H4C1 H4-161, H4C12, H4C13, H4C14, H4C15, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4FA, HIST1H4A, H4C1 H4 clustered histone 1 histone H4|H4 histone family, member A|histone 1, H4a|histone cluster 1 H4 family member a|histone cluster 1, H4a

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In this infographic, you will see the following information for H4C1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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