Product Info Summary
SKU: | PA1204 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Histamine H3 receptor HRH3 Antibody
View all Histamine H3R Antibodies
SKU/Catalog Number
PA1204
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Histamine H3 receptor HRH3 Antibody catalog # PA1204. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histamine H3 receptor HRH3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1204)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HRH3, different from the related rat sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1204 is reactive to HRH3 in Human, Mouse, Rat
Applications
PA1204 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
49 kDa
Calculated molecular weight
48.671kDa
Background of Histamine H3R
The histamine receptor H3 (HRH3) is a presynaptic autoreceptor on histamine neurons in the brain and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the central and peripheral nervous systems. The deduced 445-amino acid HRH3 protein contains 7 predicted transmembrane domains. And it shares 22% and 21.4% amino acid sequence homology with the H1 (HRH1) and H2 (HRH2) receptors, respectively. The expression of recombinant HRH3 in a variety of cell lines conferred an ability to inhibit adenylate cyclase in response to histamine, but not to acetylcholine or any other biogenic amine. Additionally, HRH3 was most notably observed throughout the thalamus, the ventromedial hypothalamus, and the caudate nucleus. Strong expression was also seen in layers II, V, and VIb of the cerebral cortex, in the pyramidal layers of the hippocampus, and in olfactory tubercle.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of HRH3 using anti-HRH3 antibody (PA1204).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates
Lane 2: human SHG-44 whole cell lysates
Lane 3: mouse RAW246.7 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HRH3 antigen affinity purified polyclonal antibody (Catalog # PA1204) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HRH3 at approximately 49KD. The expected band size for HRH3 is at 49KD.
Protein Target Info & Infographic
Gene/Protein Information For HRH3 (Source: Uniprot.org, NCBI)
Gene Name
HRH3
Full Name
Histamine H3 receptor
Weight
48.671kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
GPCR97; GPCR97G protein-coupled receptor 97; G-protein coupled receptor 97; H3R; HH3R; Histamine H3 R; histamine H3 receptor; Histamine H3R; histamine receptor H3; HRH3 HRH3 GPCR97, HH3R histamine receptor H3 histamine H3 receptor|G protein-coupled receptor 97|H3R
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HRH3, check out the HRH3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HRH3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histamine H3 receptor HRH3 Antibody (PA1204)
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No publications found for PA1204
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Histamine H3 receptor HRH3 Antibody
Question
We are currently using anti-HRH3 antibody PA1204 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-30
Answer
The anti-HRH3 antibody (PA1204) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-30