Click image to see more details
Product Info Summary
| SKU: | M00373 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC-F, CyTOF |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Hu CD24 Purified Monoclonal Antibody
SKU/Catalog Number
M00373
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD24 Purified Monoclonal Antibody (Catalog# M00373). Tested in Flow Cytometry, IHC-F, MC (CyTOF) application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD24 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00373)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
SN3
Isotype
Mouse IgG1
Immunogen
Glycoproteins purified from human NALM-1 cell line. The antibody SN3 reacts with CD24, a 35-45 kDa heavily glycosylated cell surface antigen. CD24 is expressed by granulocytes, B lymphocytes and by some activated T cells and T cell malignancies. It is not expressed on human thymocytes.
Reactive Species
M00373 is reactive to CD24 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
8.1 kDa
Background of CD24
CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-like GPI-anchored extracellular membrane glycoprotein expressed on several cell types, including B cells. When B cells are activated and induced to further maturation, however, CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins such as the chemokine receptor CXCR4; it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cell precursors but not in mature resting B cells, where it instead inhibits their ability to proliferate in response to activation. CD24 expression is associated with invasiveness and poorer prognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00373 is guaranteed for Flow Cytometry, IHC-F, CyTOF Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (frozen sections): Positive tissue: tonsil.
Flow cytometry: 1-12 µg/ml
Validation Images & Assay Conditions
Click image to see more details
Separation of human CD24 positive lymphocytes (red-filled) from CD24 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD24 (SN3) purified antibody (concentration in sample 4 µg/ml, GAM APC).
Click image to see more details
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD24 (SN3) purified antibody (concentration in sample 4 µg/ml, GAM APC).
Specific Publications For Anti-Hu CD24 Purified Monoclonal Antibody (M00373)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Hu CD24 Purified Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Hu CD24 Purified Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
