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Product Info Summary
| SKU: | M06249-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F, WB |
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Product info
Product Name
Anti-Hu CD53 Purified Monoclonal Antibody
SKU/Catalog Number
M06249-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD53 Purified Monoclonal Antibody (Catalog# M06249-1). Tested in Flow Cytometry, IP, WB, IHC-F application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD53 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06249-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
MEM-53
Isotype
Mouse IgG1
Immunogen
Leukocytes of pacient suffering from a LGL-type leukemia. The antibody MEM-53 reacts with an extracellular epitope of CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Cross-reactivity with CLIC5b was not determined.
Reactive Species
M06249-1 is reactive to CD53 in Human
Applications
M06249-1 is guaranteed for Flow Cytometry, IP, IHC-F, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
24.341kDa
Background of CD53
CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (frozen sections): It is suitable for discrimination of lymphomas from other tumors.
Flow cytometry: 1-5 μg/ml.
Validation Images & Assay Conditions
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Protein Target Info & Infographic
Gene/Protein Information For CD53 (Source: Uniprot.org, NCBI)
Gene Name
CD53
Full Name
Leukocyte surface antigen CD53
Weight
24.341kDa
Superfamily
tetraspanin (TM4SF) family
Alternative Names
antigen MOX44 identified by monoclonal MRC-OX44; CD53 antigentetraspanin-25; CD53 glycoprotein; CD53 molecule; CD53 tetraspan antigen; CD53; cell surface antigen; Cell surface glycoprotein CD53; MOX44; MOX44transmembrane glycoprotein; Tetraspanin-25; TSPAN25; tspan-25; TSPAN25leukocyte surface antigen CD53 CD53 MOX44, TSPAN25 CD53 molecule leukocyte surface antigen CD53|CD53 antigen|CD53 glycoprotein|CD53 tetraspan antigen|antigen MOX44 identified by monoclonal antibody MRC-OX44|cell surface antigen|cell surface glycoprotein CD53|tetraspanin-25|transmembrane glycoprotein|tspan-25
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD53, check out the CD53 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD53 Purified Monoclonal Antibody (M06249-1)
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No publications found for M06249-1
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