Anti-human CD22 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated

Siglec-2/CD22 antibody

Boster Bio Anti-human CD22 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Catalog# FC01572-PerCP-Cy5.5). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Product Info Summary

SKU: FC01572-PerCP-Cy5.5
Size: 100 tests, 200 tests
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

Product Name

Anti-human CD22 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated

View all Siglec-2/CD22 Antibodies

SKU/Catalog Number

FC01572-PerCP-Cy5.5

Size

100 tests, 200 tests

Form

Liquid

Description

Boster Bio Anti-human CD22 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Catalog# FC01572-PerCP-Cy5.5). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-human CD22 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01572-PerCP-Cy5.5)

Host

Mouse

Contents

PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: MYG13

Isotype

IgG1,k

Immunogen

Human Raji Cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

FC01572-PerCP-Cy5.5 is reactive to CD22 in Human

Applications

FC01572-PerCP-Cy5.5 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

95.348kDa

Background of Siglec-2/CD22

CD22 or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. This gene is mapped to 19q13.2. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. Generally speaking, CD22 is a regulatory molecule that prevents the overactivation of the immune system and the development of autoimmune diseases. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signalling. This gene can downmodulate signaling through the IgM and IgD B-cell receptors (BCRs), but not through the IgG BCR, because the IgG cytoplasmic tail prevents CD22 phosphorylation and actually enhances IgG-BCR signaling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

Gene/Protein Information For CD22 (Source: Uniprot.org, NCBI)

Gene Name

CD22

Full Name

B-cell receptor CD22

Weight

95.348kDa

Superfamily

immunoglobulin superfamily

Alternative Names

B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigenMGC130020; CD22 molecule; CD22; sialic acid binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2; Siglec2; Siglec-2; SIGLEC2FLJ22814; T-cell surface antigen Leu-14 CD22 SIGLEC-2, SIGLEC2 CD22 molecule B-cell receptor CD22|B-lymphocyte cell adhesion molecule|BL-CAM|CD22 antigen|T-cell surface antigen Leu-14|sialic acid-binding Ig-like lectin 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD22, check out the CD22 Infographic

CD22 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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10 Customer Q&As for Anti-human CD22 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated

Question

Is there a BSA free version of anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC01572-PerCP-Cy5.5 available?

Verified Customer

Verified customer

Asked: 2020-03-30

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC01572-PerCP-Cy5.5 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-30

Question

My question regarding product FC01572-PerCP-Cy5.5, anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC01572-PerCP-Cy5.5 anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-08

Question

Is a blocking peptide available for product anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated (FC01572-PerCP-Cy5.5)?

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

We do provide the blocking peptide for product anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated (FC01572-PerCP-Cy5.5). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-06

Question

We are interested in to test anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC01572-PerCP-Cy5.5 on human corpus callosum for research purposes, then I may be interested in using anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC01572-PerCP-Cy5.5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-05-20

Answer

The products we sell, including anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC01572-PerCP-Cy5.5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-20

Question

I was wanting to use your anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated for Flow Cytometry for human corpus callosum on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human corpus callosum identification?

Verified Customer

Verified customer

Asked: 2019-03-25

Answer

You can see on the product datasheet, FC01572-PerCP-Cy5.5 anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human corpus callosum in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-03-25

Question

I see that the anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC01572-PerCP-Cy5.5 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-04-03

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-04-03

Question

Would anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC01572-PerCP-Cy5.5 work for Flow Cytometry with corpus callosum?

Verified Customer

Verified customer

Asked: 2018-01-31

Answer

According to the expression profile of corpus callosum, CD22 is highly expressed in corpus callosum. So, it is likely that anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC01572-PerCP-Cy5.5 will work for Flow Cytometry with corpus callosum.

Boster Scientific Support

Answered: 2018-01-31

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for corpus callosum using anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC01572-PerCP-Cy5.5. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-08-15

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-08-15

Question

See attached the WB image, lot number and protocol we used for corpus callosum using anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC01572-PerCP-Cy5.5. Please let me know if you require anything else.

M. Anderson

Verified customer

Asked: 2015-04-17

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-04-17

Question

Will FC01572-PerCP-Cy5.5 anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

N. Johnson

Verified customer

Asked: 2014-07-17

Answer

It shows on the product datasheet, FC01572-PerCP-Cy5.5 anti-human CD22 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-07-17

Order DetailsPrice
FC01572-PerCP-Cy5.5-100tests

100 tests

$360
FC01572-PerCP-Cy5.5-200tests

200 tests

$612

Free Secondary Antibody

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FC01572-PerCP-Cy5.5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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