Anti-human CD22/Siglec 2 Monoclonal Antibody Unconjugated, Flow Validated

Product Name

Anti-human CD22/Siglec 2 Monoclonal Antibody Unconjugated, Flow Validated

View all Siglec-2/CD22 Antibodies

SKU/Catalog Number

FC01572

Size

100ug, 500ug

Form

Liquid

Description

Boster Bio Anti-human CD22/Siglec 2 Monoclonal Antibody Unconjugated, Flow Validated (Catalog# FC01572). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-human CD22/Siglec 2 Monoclonal Antibody Unconjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01572)

Host

Mouse

Contents

PBS pH 7.2, 0.1% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: MYG13

Isotype

IgG1,k

Immunogen

Human Raji Cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

FC01572 is reactive to CD22 in Human

Applications

FC01572 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

95.348kDa

Background of Siglec-2/CD22

CD22 or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. This gene is mapped to 19q13.2. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. Generally speaking, CD22 is a regulatory molecule that prevents the overactivation of the immune system and the development of autoimmune diseases. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signalling. This gene can downmodulate signaling through the IgM and IgD B-cell receptors (BCRs), but not through the IgG BCR, because the IgG cytoplasmic tail prevents CD22 phosphorylation and actually enhances IgG-BCR signaling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

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Lymphocytes gated PBMCs stained with purified anti-human CD22 (clone MYG13) followed by goat anti-mouse IgG (H+L) Alexa Fluor 488 staining (red histogram). PBMCs lymphocytes stained with purified IgG1 isotype control followed by goat antimouse IgG (H+L) Alexa Fluor 488 staining (blue histogram).

Gene/Protein Information For CD22 (Source: Uniprot.org, NCBI)

Alternative Names

B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigenMGC130020; CD22 molecule; CD22; sialic acid binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2; Siglec2; Siglec-2; SIGLEC2FLJ22814; T-cell surface antigen Leu-14 CD22 SIGLEC-2, SIGLEC2 CD22 molecule B-cell receptor CD22|B-lymphocyte cell adhesion molecule|BL-CAM|CD22 antigen|T-cell surface antigen Leu-14|sialic acid-binding Ig-like lectin 2

For more info on CD22, check out the CD22 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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