Anti-human CD5 Monoclonal Antibody PE Conjugated, Flow Validated

Product Name

Anti-human CD5 Monoclonal Antibody PE Conjugated, Flow Validated

View all CD5 Antibodies

SKU/Catalog Number

FC00480-PE

Size

100 tests, 200 tests

Form

Liquid

Description

Boster Bio Anti-human CD5 Monoclonal Antibody PE Conjugated, Flow Validated (Catalog# FC00480-PE). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-human CD5 Monoclonal Antibody PE Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00480-PE)

Host

Mouse

Contents

PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: OKT1

Isotype

IgG1,k

Immunogen

Human peripheral blood lymphocytes

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

FC00480-PE is reactive to CD5 in Human

Applications

FC00480-PE is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

54.578kDa

Background of CD5

CD5, also known as T1 or LEU1, is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. It is mapped to 11q12.2. Although CD5 can be found on both T cells and B cells, T cells express higher levels of CD5 than B cells. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. CD5 is upregulated on T cells upon strong activation. In the thymus, there is a correlation with CD5 expression and strength of the interaction of the T cell towards self-peptides. Nevertheless, CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

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Lymphocytes gated PBMCs stained with PE conjugated anti-human CD5 (clone OKT-1, red histogram). Lymphocytes gated PBMCs stained with isotype IgG1 PE (blue histogram).

Gene/Protein Information For CD5 (Source: Uniprot.org, NCBI)

Alternative Names

CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1 CD5 LEU1, T1 CD5 molecule T-cell surface glycoprotein CD5|CD5 antigen (p56-62)|epididymis secretory sperm binding protein|lymphocyte antigen T1/Leu-1

For more info on CD5, check out the CD5 Infographic

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In this infographic, you will see the following information for CD5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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