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Product Info Summary
| SKU: | FC02236-Biotin |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD8 Cd8a Monoclonal Antibody Biotin Conjugated, Flow Validated
SKU/Catalog Number
FC02236-Biotin
Size
100ug
Form
Liquid
Description
Boster Bio Anti-human CD8 Cd8a Monoclonal Antibody Biotin Conjugated, Flow Validated (Catalog# FC02236-Biotin). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD8 Cd8a Monoclonal Antibody Biotin Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC02236-Biotin)
Host
Mouse
Contents
PBS pH 7.2, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
Clone: OKT8
Isotype
IgG2a,k
Immunogen
Human peripheral blood T lymphocytes
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC02236-Biotin is reactive to CD8A in Human
Applications
FC02236-Biotin is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
25.729kDa
Background of CD8
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Lymphocytes gated PBMCs stained with biotin conjugated mouse anti-human CD8 (clone OKT8) followed by staining with SA-PE (red histogram). Lymphocytes gated PBMCs stained with isotype control mouse IgG2a biotin (clone RPC5.4) followed by SA-PE (blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For CD8A (Source: Uniprot.org, NCBI)
Gene Name
CD8A
Full Name
T-cell surface glycoprotein CD8 alpha chain
Weight
25.729kDa
Alternative Names
53-6.7 CD8 Alpha; 53-6.7 CD8 Aplha; 53-6.7 CD8; 53-6.7 Clone; 53-6.7; CD8; CD8; CD8a molecule; CD8A; Cytotoxic T cell marker; Cytotoxic T lymphocyte marker; LEU2; p32; T cell marker CD8A CD8, Leu2, p32 CD8a molecule T-cell surface glycoprotein CD8 alpha chain|CD8 antigen, alpha polypeptide (p32)|Leu2 T-lymphocyte antigen|OKT8 T-cell antigen|T cell co-receptor|T-cell antigen Leu2|T-lymphocyte differentiation antigen T8/Leu-2|T8 T-cell antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD8A, check out the CD8A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD8A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD8 Cd8a Monoclonal Antibody Biotin Conjugated, Flow Validated (FC02236-Biotin)
Hello CJ!
FC02236-Biotin has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients
Xu Y, Tan X, Wang D, Wang W, Li Y, Wu M, Chen S, Wu Y, Tan C. Int J Mol Med. 2015 Jun;35(6):1501-10. Doi: 10.3892/Ijmm.2015.2154. Epub 2015 Mar 24. Elevated Survivin Expression In Peripheral Blood Mononuclear Cells Is Central To Collateral Formati...
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-human CD8 Cd8a Monoclonal Antibody Biotin Conjugated, Flow Validated
Question
Will Anti-Human CD8 Cd8a Monoclonal Antibody Biotin Conjugated, Flow Validated (FC02236-Biotin) bind to CD8 T cells?
Verified customer
Asked: 2022-03-15
Answer
Clone OKT8, a mouse monoclonal antibody, binds with a 32-34 kDa type I transmembrane glycoprotein known as CD8 a member of the immunoglobulin superfamily. OKT8 binds to CD8α, which forms either a homodimer or a heterodimer with CD8β. It is expressed on the surface of cytotoxic T cells, NK cells, cortical thymocytes, and on some dendritic cells. CD8 functions as a co-receptor for T-cell receptors and specifically binds to class I MHC for antigen recognition and T-cell activation.
Boster Scientific Support
Answered: 2022-03-15
Question
I am interested in to test anti-human CD8 Monoclonal antibody Biotin Conjugated, Flow Validated FC02236-Biotin on human thymus for research purposes, then I may be interested in using anti-human CD8 Monoclonal antibody Biotin Conjugated, Flow Validated FC02236-Biotin for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The products we sell, including anti-human CD8 Monoclonal antibody Biotin Conjugated, Flow Validated FC02236-Biotin, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-27
Question
I was wanting to use your anti-human CD8 Monoclonal antibody Biotin Conjugated, Flow Validated for Flow Cytometry for human thymus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human thymus identification?
Verified Customer
Verified customer
Asked: 2019-02-01
Answer
You can see on the product datasheet, FC02236-Biotin anti-human CD8 Monoclonal antibody Biotin Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-01
