Anti-Human IBA1 AIF1 Antibody

Boster Bio Anti-Human IBA1 AIF1 Antibody catalog # M01394-3. Tested in IF, IHC, WB applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: M01394-3
Size: 100ul
Reactive Species: Human
Host: Chicken
Application: IF, IHC, WB

Product Name

Anti-Human IBA1 AIF1 Antibody

See all AIF-1/Iba1 products

SKU/Catalog Number

M01394-3

Size

100ul

Description

Boster Bio Anti-Human IBA1 AIF1 Antibody catalog # M01394-3. Tested in IF, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at 4℃

Cite This Product

Anti-Human IBA1 AIF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01394-3)

Host

Chicken

Contents

Supplied as an aliquot of IgY preparation plus 5mM NaN3

Clonality

Polyclonal

Immunogen

Peptide identical to part of the C-terminal of human IBA1 coupled to KLH

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01394-3 is reactive to AIF1 in Human

Applications

M01394-3 is guaranteed for IF, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB: 1:1,000-5,000. IF 1:2,000-5,000

Validation Images & Assay Conditions

Gene/Protein Information For AIF1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P55008

Gene ID

199

Gene Name

AIF1

Full Name

Allograft inflammatory factor 1

Weight

16.703kDa

Alternative Names

AIF1; AIF-1; AIF-1IRT1; allograft inflammatory factor 1; G1; IBA1; IBA1Em:AF129756.17; IbaI; interferon gamma responsive transcript; Ionized calcium-binding adapter molecule 1; IRT1; IRT-1; Protein G1 AIF1 AIF-1, IBA1, IRT-1, IRT1 allograft inflammatory factor 1 allograft inflammatory factor 1|interferon gamma responsive transcript|ionized calcium-binding adapter molecule 1|protein G1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on AIF1, check out the AIF1 Infographic

AIF1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for AIF1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M01394-3 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5a/PKC Signaling Pathway

Mechanism of apoptosis induction in human hepatocellular carcinoma cells following treatment with a gecko peptides mixture

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5 Customer Q&As for Anti-Human IBA1 AIF1 Antibody

Question

We have been able to see staining in human metanephric glomerulus. Are there any suggestions? Is anti-Human IBA1 antibody supposed to stain metanephric glomerulus positively?

Verified Customer

Verified customer

Asked: 2019-12-31

Answer

From what I have seen in literature metanephric glomerulus does express AIF1. From what I have seen in Uniprot.org, AIF1 is expressed in metanephric glomerulus, lymphocyte, glial tumor, prostate, erythroleukemia, liver, among other tissues. Regarding which tissues have AIF1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Glial tumor, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymphocyte, Pubmed ID: 8912632
Prostate, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-12-31

Question

We are currently using anti-Human IBA1 antibody M01394-3 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

The anti-Human IBA1 antibody (M01394-3) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-25

Question

My boss were content with the WB result of your anti-Human IBA1 antibody. However we have been able to see positive staining in liver cytoskeleton using this antibody. Is that expected? Could you tell me where is AIF1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-01-08

Answer

According to literature, liver does express AIF1. Generally AIF1 expresses in cytoplasm, cytoskeleton. Regarding which tissues have AIF1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Glial tumor, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymphocyte, Pubmed ID: 8912632
Prostate, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-01-08

Question

We ordered your anti-Human IBA1 antibody for WB on lymphocyte in the past. I am using human, and We are going to use the antibody for IHC next. My question regards examining lymphocyte as well as metanephric glomerulus in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2018-10-02

Answer

I took a look at the website and datasheets of our anti-Human IBA1 antibody and it appears that M01394-3 has been tested on human in both WB and IHC. Thus M01394-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-10-02

Question

I am interested in using your anti-Human IBA1 antibody for response to electrical stimulus studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.

J. Gonzalez

Verified customer

Asked: 2014-07-24

Answer

Thanks for your inquiry. This M01394-3 anti-Human IBA1 antibody is tested on mouse brain, granule cells, purkinje cells. It is guaranteed to work for IF, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2014-07-24

$299

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M01394-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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