Anti-Human IL-17E Biotin Conjugated IL25 Monoclonal Antibody

Product Name

Anti-Human IL-17E Biotin Conjugated IL25 Monoclonal Antibody

View all IL-17E/IL-25 Antibodies

SKU/Catalog Number

B04429

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-Human IL-17E Biotin Conjugated IL25 Monoclonal Antibody (Catalog # B04429). Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at 4°C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Human IL-17E Biotin Conjugated IL25 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # B04429)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 11K8B1 IgG1

Isotype

IgG1

Immunogen

Anti-IL-17E (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17E produced in E.coli followed by hybridoma development.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

B04429 is reactive to IL25 in Human

Applications

B04429 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

20.33kDa

Background of IL-17E/IL-25

Anti-IL17E recognizes IL-17E (also known as IL25). IL-17E is produced and secreted by intra-epithelial lymphocytes, lung epithelial cells, alveolar macrophages, eosinophils, basophils, NKT cells, mast cells and cells of the gastro-intestinal tract and uterus. IL-17E stimulates the production of Th2-type cytokines. IL-17E limits Th17 cell development by inducing the expression of IL-13 by dendritic cells or by inhibiting IL-23 production by macrophages. Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. A number of cytokines, including IL-17E, have been implicated as oncogenes, reflecting the importance of these proteins in cell growth and survival. Anti-IL-17E cytokine antibody is ideal for investigators involved in Immunology, Signal Transduction research, Cancer and pro-inflammatory pathologies.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
Flow Cytometry: 1µg/mL
Fluorochrome Protein Value: 10-20
WB: 0.5µg/mL

Validation Images & Assay Conditions

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Flow cytometry analysis of PC3 cells expressing IL-17E using 1 μg of mouse anti-IL-17E monoclonal antibody (Catalog # B04429) (red line) or control (green line). Anti-mouse IgG PE conjugated secondary antibodywas used to detect the primary antibody.

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Western blot analysis of IL-17E expression in full length recombinant protein. IL-17E was detected using mouse anti-IL-17E antibody monoclonal antibody (Catalog # B04429) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).

Gene/Protein Information For IL25 (Source: Uniprot.org, NCBI)

Alternative Names

IL17E; IL-17E; IL25; IL-25; interleukin 25; Interleukin-17E; interleukin-25 IL25 IL17E interleukin 25 interleukin-25|interleukin-17E

For more info on IL25, check out the IL25 Infographic

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In this infographic, you will see the following information for IL25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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