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Product Info Summary
| SKU: | A01160 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-IL-11 Monoclonal Antibody
SKU/Catalog Number
A01160
Size
100μl
Form
Liquid
Description
Boster Bio Anti-IL-11 Monoclonal Antibody catalog # A01160. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL-11 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01160)
Host
Mouse
Contents
Purified antibody in PBS containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
2D3
Isotype
IgG
Immunogen
Purified recombinant fragment of human IL-11 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01160 is reactive to IL11 in Human
Applications
A01160 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
21.429kDa
Background of IL-11
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development
Scheid MP,et al. (2005)Mol Cell Biol; 25(6): 2347-63
Chen H, et al. (2001) Biochemistry; 40(39): 11851-9
Sato S,et al. (2002) J Biol Chem; 277(42): 39360-7
Lim MA, et al.(2003)Proc Natl Acad Sci U S A; 100(24): 14006-11
Beausoleil SA, et al. (2004)Proc Natl Acad Sci U S A; 101(33): 12130-5
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis using IL-11 Monoclonal antibody against truncated IL-11 recombinant protein.
Protein Target Info & Infographic
Gene/Protein Information For IL11 (Source: Uniprot.org, NCBI)
Gene Name
IL11
Full Name
Interleukin-11
Weight
21.429kDa
Superfamily
IL-6 superfamily
Alternative Names
Adipogenesis inhibitory factor; AGIF; AGIFoprelvekin; IL11; IL-11; IL-11Oprelvekin; interleukin 11; interleukin-11; Oprelvekin IL11 AGIF, IL-11 interleukin 11 interleukin-11|adipogenesis inhibitory factor|oprelvekin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL11, check out the IL11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-11 Monoclonal Antibody (A01160)
Hello CJ!
A01160 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cas9 Protein Triggers Differential Expression of Inherent Genes Especially NGFR Expression in 293T Cells
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-IL-11 Monoclonal Antibody
Question
I see that the anti-IL-11 Monoclonal antibody A01160 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-15
Question
Is there a BSA free version of anti-IL-11 Monoclonal antibody A01160 available?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IL-11 Monoclonal antibody A01160 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-23
Question
My colleagues were happy with the WB result of your anti-IL-11 Monoclonal antibody . However we have seen positive staining in adenohypophysis secreted using this antibody. Is that expected? Could you tell me where is IL11 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
Based on literature, adenohypophysis does express IL11. Generally IL11 expresses in secreted. Regarding which tissues have IL11 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 1828438
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-02-14
Question
We have been able to see staining in human adenohypophysis. Do you have any suggestions? Is anti-IL-11 Monoclonal antibody supposed to stain adenohypophysis positively?
S. Rodriguez
Verified customer
Asked: 2020-01-08
Answer
From literature adenohypophysis does express IL11. From Uniprot.org, IL11 is expressed in adenohypophysis, bone marrow, prostate, among other tissues. Regarding which tissues have IL11 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 1828438
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-08
Question
I am interested in to test anti-IL-11 Monoclonal antibody A01160 on human bone marrow for research purposes, then I may be interested in using anti-IL-11 Monoclonal antibody A01160 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Williams
Verified customer
Asked: 2019-07-16
Answer
The products we sell, including anti-IL-11 Monoclonal antibody A01160, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-16
Question
Is this A01160 anti-IL-11 Monoclonal antibody reactive to the isotypes of IL11?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The immunogen of A01160 anti-IL-11 Monoclonal antibody is Purified recombinant fragment of human IL-11 expressed in E. Coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-IL-11 Monoclonal antibody A01160. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-21
Question
Here is the WB image, lot number and protocol we used for bone marrow using anti-IL-11 Monoclonal antibody A01160. Please let me know if you require anything else.
B. Carter
Verified customer
Asked: 2019-03-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-04
Question
Is a blocking peptide available for product anti-IL-11 Monoclonal antibody (A01160)?
Verified Customer
Verified customer
Asked: 2019-02-19
Answer
We do provide the blocking peptide for product anti-IL-11 Monoclonal antibody (A01160). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-19
Question
My question regarding product A01160, anti-IL-11 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Taylor
Verified customer
Asked: 2017-06-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01160 anti-IL-11 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-27
Question
We are currently using anti-IL-11 Monoclonal antibody A01160 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
B. Williams
Verified customer
Asked: 2016-02-24
Answer
The anti-IL-11 Monoclonal antibody (A01160) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-02-24
Question
Will A01160 anti-IL-11 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Lewis
Verified customer
Asked: 2015-12-09
Answer
As indicated on the product datasheet, A01160 anti-IL-11 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-12-09
Question
I was wanting to use your anti-IL-11 Monoclonal antibody for WB for human bone marrow on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human bone marrow identification?
A. Banerjee
Verified customer
Asked: 2014-12-05
Answer
As indicated on the product datasheet, A01160 anti-IL-11 Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-05
Question
Will anti-IL-11 Monoclonal antibody A01160 work for WB with bone marrow?
R. Wu
Verified customer
Asked: 2013-08-19
Answer
According to the expression profile of bone marrow, IL11 is highly expressed in bone marrow. So, it is likely that anti-IL-11 Monoclonal antibody A01160 will work for WB with bone marrow.
Boster Scientific Support
Answered: 2013-08-19
