Click image to see more details
Product Info Summary
| SKU: | PA1529 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Interleukin-4 IL4 Antibody
SKU/Catalog Number
PA1529
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interleukin-4 IL4 Antibody catalog # PA1529. Tested in WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-4 IL4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1529)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse IL-4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1529 is reactive to IL4 in Mouse
Applications
PA1529 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
37 kDa
Calculated molecular weight
17.492kDa
Background of IL-4
Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo, in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.2 The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-IL-4 antibody, PA1529, Western blotting
Lane 1: Recombinant Mouse IL-4 Protein 10ng
Lane 2: Recombinant Mouse IL-4 Protein 5ng
Lane 3: Recombinant Mouse IL-4 Protein 2.5ng
Protein Target Info & Infographic
Gene/Protein Information For IL4 (Source: Uniprot.org, NCBI)
Gene Name
IL4
Full Name
Interleukin-4
Weight
17.492kDa
Superfamily
IL-4/IL-13 family
Alternative Names
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra IL4 BCGF-1, BCGF1, BSF-1, BSF1, IL-4 interleukin 4 interleukin-4|B cell growth factor 1|B_cell stimulatory factor 1|binetrakin|interleukin 4 variant 2|lymphocyte stimulatory factor 1|pitrakinra
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL4, check out the IL4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Interleukin-4 IL4 Antibody (PA1529)
Hello CJ!
PA1529 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.
Tan Y, Liu H, Yang H, Wang L, Qin X. J Physiol Sci. 2013 Jan;63(1):63-9. Doi: 10.1007/S12576-012-0231-X. Epub 2012 Sep 26. An Inactivated Pseudomonas Aeruginosa Medicament Inhibits Airway Allergic Inflammation And Improves Epithelial Functions.
Wang Yz, Feng Xg, Shi Qg, Hao Yl, Yang Y, Zhang Am, Kong Qx. Inflammation. 2013 Apr;36(2):337-45. Doi: 10.1007/S10753-012-9551-5. Silencing Of Mir155 Promotes The Production Of Inflammatory Mediators In Guillain-Barr?? Syndrome In Vitro.
Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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3 Customer Q&As for Anti-Interleukin-4 IL4 Antibody
Question
We have been able to see staining in mouse oocyte. Do you have any suggestions? Is anti-IL4 antibody supposed to stain oocyte positively?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
From what I have seen in literature oocyte does express IL4. From what I have seen in Uniprot.org, IL4 is expressed in oocyte, blood, among other tissues. Regarding which tissues have IL4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-17
Question
Our lab were content with the WB result of your anti-IL4 antibody. However we have seen positive staining in oocyte secreted. using this antibody. Is that expected? Could you tell me where is IL4 supposed to be expressed?
M. Parker
Verified customer
Asked: 2016-08-04
Answer
Based on literature, oocyte does express IL4. Generally IL4 expresses in secreted. Regarding which tissues have IL4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-08-04
Question
We are currently using anti-IL4 antibody PA1529 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on primate tissues as well?
T. Kulkarni
Verified customer
Asked: 2013-10-04
Answer
The anti-IL4 antibody (PA1529) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-10-04
