Anti-Integrin alpha V ITGAV Rabbit Monoclonal Antibody

Product Name

Anti-Integrin alpha V ITGAV Rabbit Monoclonal Antibody

View all ITGAV Antibodies

SKU/Catalog Number

M01561

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Integrin alpha V ITGAV Rabbit Monoclonal Antibody catalog # M01561. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Integrin alpha V ITGAV Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01561)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ABGC-9

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Integrin alpha V

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01561 is reactive to ITGAV in Human, Mouse, Rat

Applications

M01561 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

69 kDa

Calculated molecular weight

116.038kDa

Background of ITGAV

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:40

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ITGAV using anti-ITGAV antibody (M01561).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGAV antigen affinity purified monoclonal antibody (Catalog # M01561) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGAV at approximately 130, 140, 150 kDa. The expected band size for ITGAV is at 116 kDa.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

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Immunofluorescent analysis using the Antibody at 1:150 dilution.

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Immunofluorescent analysis using the Antibody at 1:500 dilution.

Gene/Protein Information For ITGAV (Source: Uniprot.org, NCBI)

Alternative Names

CD51; Integrin alpha V beta 5; integrin subunit alpha V; ITGAV; MSK8; VNRA; VTNR ITGAV CD51, MSK8, VNRA, VTNR integrin subunit alpha V integrin alpha-V|antigen identified by monoclonal antibody L230|integrin alphaVbeta3|integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)|vitronectin receptor subunit alpha

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In this infographic, you will see the following information for ITGAV: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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