Product Info Summary
SKU: | M02848-2 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-K63-linkage Specific Ubiquitin UBB Rabbit Monoclonal Antibody
SKU/Catalog Number
M02848-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-K63-linkage Specific Ubiquitin UBB Rabbit Monoclonal Antibody catalog # M02848-2. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-K63-linkage Specific Ubiquitin UBB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02848-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IGC-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human K63-linkage Specific Ubiquitin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M02848-2 is reactive to UBB in Human, Mouse, Rat
Applications
M02848-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
55 kDa
Calculated molecular weight
25.762kDa
Background of UBB
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Ubiquitin expression in K63-linked-Ub2 recombinant protein.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human tonsil, using K63-linkage Specific Ubiquitin Antibody.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For UBB (Source: Uniprot.org, NCBI)
Gene Name
UBB
Full Name
Polyubiquitin-B
Weight
25.762kDa
Superfamily
ubiquitin family
Alternative Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin UBB HEL-S-50 ubiquitin B polyubiquitin-B|epididymis secretory protein Li 50|polyubiquitin B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBB, check out the UBB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-K63-linkage Specific Ubiquitin UBB Rabbit Monoclonal Antibody (M02848-2)
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No publications found for M02848-2
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-K63-linkage Specific Ubiquitin UBB Rabbit Monoclonal Antibody
Question
My team were well pleased with the WB result of your anti-K63-linkage Specific Ubiquitin Rabbit Monoclonal antibody. However we have seen positive staining in hypothalamus ubiquitin: cytoplasm. nucleus using this antibody. Is that expected? Could you tell me where is UBB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
From literature, hypothalamus does express UBB. Generally UBB expresses in ubiquitin: cytoplasm. nucleus. Regarding which tissues have UBB expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3029682
Brain, Liver, and Lung, Pubmed ID: 15489334
Fetal brain cortex, Pubmed ID: 1128706
Boster Scientific Support
Answered: 2020-03-09
Question
We have tried in the past anti-K63-linkage Specific Ubiquitin Rabbit Monoclonal antibody for IF on hypothalamus in the past. I am using human, and We want to use the antibody for WB next. I am looking for examining hypothalamus as well as liver lung in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
I looked at the website and datasheets of our anti-K63-linkage Specific Ubiquitin Rabbit Monoclonal antibody and I see that M02848-2 has been validated on human in both IF and WB. Thus M02848-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-19
Question
We are currently using anti-K63-linkage Specific Ubiquitin Rabbit Monoclonal antibody M02848-2 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-12
Answer
The anti-K63-linkage Specific Ubiquitin Rabbit Monoclonal antibody (M02848-2) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-12
Question
We have observed staining in human blood. What should we do? Is anti-K63-linkage Specific Ubiquitin Rabbit Monoclonal antibody supposed to stain blood positively?
A. Krishna
Verified customer
Asked: 2013-11-07
Answer
From literature blood does express UBB. From Uniprot.org, UBB is expressed in hypothalamus, blood, brain, liver lung, fetal brain cortex, among other tissues. Regarding which tissues have UBB expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3029682
Brain, Liver, and Lung, Pubmed ID: 15489334
Fetal brain cortex, Pubmed ID: 1128706
Boster Scientific Support
Answered: 2013-11-07