Product Info Summary
SKU: | A02305 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-Keratin 10 KRT10 Antibody
SKU/Catalog Number
A02305
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Keratin 10 KRT10 Antibody (Catalog # A02305). Tested in WB, IF applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Keratin 10 KRT10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02305)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Keratin 10.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02305 is reactive to KRT10 in Human, Rat
Applications
A02305 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
58.827kDa
Background of KRT10
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.
XM Zhou, J. Biol. Chem., Oct 1988; 263: 15584 - 15589.
Felix B. M¨¹ller, Hum. Mol. Genet., Apr 2006; 15: 1133 - 1141.
J Reichelt, J. Cell Sci., Sep 1997; 110: 2175.
BP Korge, PNAS, Feb 1992; 89: 910.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from HeLa cells, using Keratin 10 antibody A02305.
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Western blot analysis of extracts from COS7 cells and 3T3 cells using Keratin 10 antibody A02305. The lane on the left is treated with synthesized peptide.
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Immunofluorescence analysis of A549 cells, using Keratin 10 antibody A02305.
Protein Target Info & Infographic
Gene/Protein Information For KRT10 (Source: Uniprot.org, NCBI)
Gene Name
KRT10
Full Name
Keratin, type I cytoskeletal 10
Weight
58.827kDa
Superfamily
intermediate filament family
Alternative Names
BCIE; BIE; CK10; CK-10; cytokeratin 10; Cytokeratin-10; EHK; K10keratosis palmaris et plantaris; Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris); keratin 10; keratin, type I cytoskeletal 10; keratin-10; KPP KRT10 BCIE, BIE, CK10, EHK, K10, KPP keratin 10 keratin, type I cytoskeletal 10|CK-10|cytokeratin 10|keratin 10, type I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT10, check out the KRT10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Keratin 10 KRT10 Antibody (A02305)
Hello CJ!
A02305 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Localization of Human Mesenchymal Stem Cells from Umbilical Cord Blood and Their Role in Repair of Diabetic Foot Ulcers in Rats
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