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- Table of Contents
KRT17 antibody (M02289-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of Cytokeratin-17 using anti-Cytokeratin-17 antibody (M02289-1).
Cytokeratin-17 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Cytokeratin-17 Antibody (M02289-1) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Cytokeratin-17 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Cytokeratin-17 Antibody (M02289-1) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Cytokeratin 17 Rabbit Monoclonal Antibody
KRT17 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
KRT17 antibody was positively detected by WB in:
human Hela whole cell , human A431 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
KRT17 antibody was positively detected by IHC in:
human bladder cancer tissue, human breast cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
KRT17 antibody was positively detected by ICC/IF in:
Hela cell
KRT17 antibody was positively detected by IF in:
human bladder cancer tissue, human lung cancer tissue
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:30
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
FC
Human
Tested
Tested
Tested
Recommend
Mouse
Recommend
Rat
Recommend
Product Information
Product Name
Anti-Cytokeratin 17 Rabbit Monoclonal Antibody
View all KRT17 Antibodies
View all KRT17 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DIB-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytokeratin 17
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
48 kDa
Calculated Molecular Weight
48.1 kDa
Cite This Product
Anti-Cytokeratin 17 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02289-1)
Protocols
Western Blot protocol for KRT17 antibody
Immunohistochemistry (IHC) protocol for KRT17 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for KRT17 antibody
Immunofluorescence (IF) protocol for KRT17 antibody
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Product Promise
We promise Anti-Cytokeratin 17 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M02289-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026