Product Info Summary
SKU: | M02101-5 |
---|---|
Size: | 50 µl |
Reactive Species: | Human |
Host: | Mouse |
Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-KRT19 Antibody
View all Cytokeratin 19 Antibodies
SKU/Catalog Number
M02101-5
Size
50 µl
Description
Boster Bio Anti-KRT19 Antibody (Catalog # M02101-5). Tested in IHC-P, IF, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-KRT19 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02101-5)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
979CT14.3.1
Isotype
IgG1,k
Immunogen
This antibody is generated from a mouse immunized with a recombinant protein of human.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02101-5 is reactive to KRT19 in Human
Applications
M02101-5 is guaranteed for IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
44.106kDa
Background of Cytokeratin 19
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:8000
IHC: 1:400
IHC-P: 1:25
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling KRT19 with M02101-5 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing intermediate filaments staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).
Click image to see more details
All lanes : Anti-KRT19 at 1:8000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 44 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human Colon cancer section using Pink1. M02101-5 was diluted at 1:400 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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M02101-5 staining KRT19 in human kidney sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For KRT19 (Source: Uniprot.org, NCBI)
Gene Name
KRT19
Full Name
Keratin, type I cytoskeletal 19
Weight
44.106kDa
Superfamily
intermediate filament family
Alternative Names
40-kDa keratin intermediate filament; CK19; CK-19; Cytokeratin 19; EndoC; K19; K1CS; keratin 19; keratin, type I cytoskeletal 19; keratin, type I, 40-kd; keratin-19; Krt19; MGC15366 KRT19 CK19, K19, K1CS keratin 19 keratin, type I cytoskeletal 19|40-kDa keratin intermediate filament|CK-19|cytokeratin 19|keratin 19, type I|keratin, type I, 40-kd
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT19, check out the KRT19 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KRT19 Antibody (M02101-5)
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No publications found for M02101-5
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