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Product Info Summary
| SKU: | M00479 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Leptin Monoclonal Antibody
SKU/Catalog Number
M00479
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Leptin Monoclonal Antibody catalog # M00479. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Leptin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00479)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
5C11-G6-B12
Isotype
IgG
Immunogen
Purified recombinant full length of human Leptin protein expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00479 is reactive to LEP in Human
Applications
M00479 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
18.641kDa
Background of Leptin/OB
Paired box protein Pax-6 also known as aniridia type II protein (AN2) or oculorhombin is a transcription factor with key functions in the development of the eye, nose, central nervous system and pancreas. Pax6 further seems to be required for the differentiation of pancreatic islet alpha cells. PAX6 has been shown to bind to a promoter element in the glucagon, insulin and somatostatin promoters and regulates the specification of the ventral neuron subtypes by establishing the correct progenitor domains. Isoform 5a appears to function as a molecular switch that specifies target genes. Anti-PAX6 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ELISA 1:10000-20000
Validation Images & Assay Conditions
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Figure 1. ELISA validation of LEP using Anti-Leptin Monoclonal Antibody (M00479).
Indirect ELISA assay for anti-Leptin mouse mAb. Antigen coating concentration:4ug/ml.Protein Target Info & Infographic
Gene/Protein Information For LEP (Source: Uniprot.org, NCBI)
Gene Name
LEP
Full Name
Leptin
Weight
18.641kDa
Superfamily
leptin family
Alternative Names
FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS LEP LEPD, OB, OBS leptin leptin|leptin (murine obesity homolog)|leptin (obesity homolog, mouse)|obese protein|obese, mouse, homolog of|obesity factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LEP, check out the LEP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LEP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Leptin Monoclonal Antibody (M00479)
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No publications found for M00479
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Customer Reviews
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