Anti-LPAAT- theta GPAT3 Antibody

GPAT3 antibody

Boster Bio Anti-LPAAT- theta GPAT3 Antibody catalog # A07965. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A07965
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-LPAAT- theta GPAT3 Antibody

View all GPAT3 Antibodies

SKU/Catalog Number

A07965

Size

100μl

Form

Liquid

Description

Boster Bio Anti-LPAAT- theta GPAT3 Antibody catalog # A07965. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LPAAT- theta GPAT3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07965)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

1A3

Isotype

IgG

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human PABPC4

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07965 is reactive to GPAT3 in Human, Mouse, Rat

Applications

A07965 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

48.705kDa

Background of GPAT3

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation

Feng Cong, et,al. (1999) Mol. Cell. Biol; 19: 8314 - 8325.
Serge Lemay, et,al. (2000) Mol. Cell. Biol; 20: 2743 - 2754.
Ute Schaeper, et,al.(2000) J. Cell Biol; 149: 1419.
Miyuki Honma, et,al. (2006) Genes Cells; 11: 143 - 151.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For GPAT3 (Source: Uniprot.org, NCBI)

Gene Name

GPAT3

Full Name

Glycerol-3-phosphate acyltransferase 3

Weight

48.705kDa

Superfamily

1-acyl-sn-glycerol-3-phosphate acyltransferase family

Alternative Names

Glycerol-3-phosphate acyltransferase 3 GPAT3 AGPAT 10, AGPAT10, AGPAT8, AGPAT9, HMFN0839, LPAAT-theta, MAG1 glycerol-3-phosphate acyltransferase 3 glycerol-3-phosphate acyltransferase 3|1-AGP acyltransferase 9|1-AGPAT 9|1-acyl-sn-glycerol-3-phosphate O-acyltransferase 10|1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9|1-acylglycerol-3-phosphate O-acyltransferase 8|1-acylglycerol-3-phosphate O-acyltransferase 9|GPAT-3|MAG-1|acyl-CoA:glycerol-3-phosphate acyltransferase 3|endoplasmic reticulum associated GPAT|hGPAT3|lung cancer metastasis-associated protein 1|lysophosphatidic acid acyltransferase theta|testis secretory sperm-binding protein Li 213e

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GPAT3, check out the GPAT3 Infographic

GPAT3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GPAT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-LPAAT- theta GPAT3 Antibody

Question

Can you help my question with product A07965, anti-LPAAT- theta antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-21

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07965 anti-LPAAT- theta antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-21

Question

Will anti-LPAAT- theta antibody A07965 work for WB with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

According to the expression profile of cervix carcinoma, GPAT3 is highly expressed in cervix carcinoma. So, it is likely that anti-LPAAT- theta antibody A07965 will work for WB with cervix carcinoma.

Boster Scientific Support

Answered: 2019-12-19

Question

I was wanting to use your anti-LPAAT- theta antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2019-05-30

Answer

As indicated on the product datasheet, A07965 anti-LPAAT- theta antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-30

Question

Do you have a BSA free version of anti-LPAAT- theta antibody A07965 available?

Verified Customer

Verified customer

Asked: 2019-05-22

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-LPAAT- theta antibody A07965 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-05-22

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-LPAAT- theta antibody A07965. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-11-22

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-22

Question

Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-LPAAT- theta antibody A07965. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-08-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-08-03

Question

Does anti-LPAAT- theta antibody A07965 work on bovine WB with pns testis?

Verified Customer

Verified customer

Asked: 2017-09-26

Answer

Our lab technicians have not tested anti-LPAAT- theta antibody A07965 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-LPAAT- theta antibody A07965 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine pns testis in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-09-26

Order DetailsPrice
A07965

100uL

$399

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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