Anti-Macrosialin CD68 Monoclonal Antibody

Product Name

Anti-Macrosialin CD68 Monoclonal Antibody

View all CD68/SR-D1 Antibodies

SKU/Catalog Number

M00602-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Macrosialin CD68 Monoclonal Antibody catalog # M00602-2. Tested in IF, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Macrosialin CD68 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00602-2)

Host

Mouse

Contents

PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide and 50% Glycerol.

Clonality

Monoclonal

Clone Number

6F3

Isotype

IgG

Immunogen

Synthetic Peptide

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00602-2 is reactive to CD68 in Human, Mouse, Rat

Applications

M00602-2 is guaranteed for IF, IHC-P Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

37.408kDa

Background of CD68/SR-D1

CD68, cluster of differentiation, is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. The CD68 gene is mapped to 17p13.1. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia (the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC 1:200
IF 1:50-200

Validation Images & Assay Conditions

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IHC staining of human tonsil tissue, diluted at 1:200.

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Immunofluorescence analysis of Mouse-spleen tissue. 1, CD68 monoclonal antibody (6F3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, CD68 monoclonal antibody (6F3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

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Immunofluorescence analysis of Human-lung-cancer tissue. 1, CD68 monoclonal antibody (6F3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, CD68 monoclonal antibody (6F3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Gene/Protein Information For CD68 (Source: Uniprot.org, NCBI)

Alternative Names

CD68 antigenmacrophage antigen CD68; CD68 molecule; CD68; DKFZp686M18236; gp110; Macrosialin; SCARD1; scavenger receptor class D, member 1; SRD1; SR-D1 CD68 GP110, LAMP4, SCARD1 CD68 molecule macrosialin|CD68 antigen|macrophage antigen CD68|scavenger receptor class D, member 1

For more info on CD68, check out the CD68 Infographic

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In this infographic, you will see the following information for CD68: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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