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Product Info Summary
| SKU: | A04842-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MAP3K4 Antibody
SKU/Catalog Number
A04842-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MAP3K4 Antibody catalog # A04842-1. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MAP3K4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04842-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human HMG-17 around the phosphorylation site of S29.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04842-1 is reactive to MAP3K4 in Human, Mouse, Rat
Applications
A04842-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
181.685kDa
Background of MEKK4
Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.
Kotaka M., J. Cell. Biochem. 72:279-285(1999).
Bauer K., Blood 96:4236-4245(2000).
Deloukas P., Nature 429:375-381(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
Validation Images & Assay Conditions
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Figure 1. Western blotting validation for Anti-MAP3K4 Antibody A04842-1
Western blot (WB) analysis of MAP3K4 polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysate
Lane2:HEK293T whole cell lysate
Lane3:PC12 whole cell lysate
Lane4:SP20 whole cell lysate
Lane5:H9C2 whole cell lysate
Lane6:Raw264.7 whole cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For MAP3K4 (Source: Uniprot.org, NCBI)
Gene Name
MAP3K4
Full Name
Mitogen-activated protein kinase kinase kinase 4
Weight
181.685kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11.25; FLJ42439; MAPKKK4yeast, homolog of; MEKK 4; mitogen-activated protein kinase kinase kinase 4 MAP3K4 MAPKKK4, MEKK 4, MEKK4, MTK1, PRO0412 mitogen-activated protein kinase kinase kinase 4 mitogen-activated protein kinase kinase kinase 4|MAP three kinase 1|MAP/ERK kinase kinase 4|MAPK/ERK kinase kinase 4|MEK kinase 4|SSK2/SSK22 MAP kinase kinase kinase, yeast, homolog of|dJ473J16.1 (mitogen-activated protein kinase kinase kinase 4)|predicted protein of HQ0412
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP3K4, check out the MAP3K4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP3K4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAP3K4 Antibody (A04842-1)
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No publications found for A04842-1
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