Anti-MLX Antibody Picoband™

Product Name

Anti-MLX Antibody Picoband™

View all MLX Antibodies

SKU/Catalog Number

A03087-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MLX Antibody Picoband™ catalog # A03087-3. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-MLX Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03087-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MLX recombinant protein (Position: R33-Y298).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03087-3 is reactive to MLX in Human

Applications

A03087-3 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

33 kDa

Calculated molecular weight

33.3kDa

Background of MLX

Max-like protein X is a protein that in humans is encoded by the MLX gene. The product of this gene belongs to the family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. These factors form heterodimers with Mad proteins and play a role in proliferation, determination and differentiation. This gene product may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors, namely, Mad1 and Mad4. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml/ml, Human
Direct ELISA, 0.1-0.5 μg/ml/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of MLX using anti-MLX antibody (A03087-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLX antigen affinity purified polyclonal antibody (Catalog # A03087-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLX at approximately 33 kDa. The expected band size for MLX is at 30 kDa.

Gene/Protein Information For MLX (Source: Uniprot.org, NCBI)

Alternative Names

BHLHD13; bHLHd13Max-like bHLHZip protein; BigMax alpha; BigMax protein; Class D basic helix-loop-helix protein 13; MAD7; MAX-like protein X; MLX; MXD7; TCFL4; TCFL4Protein BigMax; transcription factor-like 4; Transcription factor-like protein 4 MLX MAD7, MXD7, TCFL4, TF4, bHLHd13 MAX dimerization protein MLX max-like protein X|BigMax protein|MAX-like bHLHZIP protein|MLX, MAX dimerization protein|class D basic helix-loop-helix protein 13|transcription factor-like protein 4

For more info on MLX, check out the MLX Infographic

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In this infographic, you will see the following information for MLX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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