Anti-Mouse IL-17A Monoclonal Antibody

Product Name

Anti-Mouse IL-17A Monoclonal Antibody

View all IL-17/IL-17A Antibodies

SKU/Catalog Number

M00421

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Mouse IL-17A Monoclonal Antibody (Catalog # M00421). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store IL-17A antibody at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Mouse IL-17A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00421)

Host

Rat

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 20B4.G10.F5 IgG2a kappa

Isotype

IgG2a kappa

Immunogen

This Mouse IL-17A monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse IL-17A protein (produced in E.coli) followed by hybridoma development.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00421 is reactive to IL17A in Mouse

Applications

M00421 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

19 kDa

Calculated molecular weight

17.504kDa

Background of IL-17/IL-17A

Mouse Interleukin-17A (IL-17A), also known as CTLA-8, is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Mouse IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58% identity between the amino acid sequence of human and mouse IL-17A. Anti-IL-17A antibody is ideal for investigators involved in Immunology research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000
Flow Cytometry: 1:500
WB: 1:1000

Validation Images & Assay Conditions

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Monoclonal anti IL-17A was used to detect IL17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 μg/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 μg/mL anti-mouse IFN over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 μg/mL, washed, fixed and permeabilized and incubated with rat anti mouse IL-17A monoclonal Antibody (Catalog # M00421) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry.

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Western blot analysis of Mouse IL-17A using rat anti mouse IL-17A monoclonal Antibody (Catalog # M00421) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002). Band indicates correct 23 kDa molecular weight position expected for Mouse IL-17A.

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Monoclonal anti IL-17A was used to detect IL17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 μg/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 μg/mL anti-mouse IFN over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 μg/mL, washed, fixed and permeabilized and incubated with rat anti mouse IL-17A monoclonal Antibody (Catalog # M00421) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry.

Click image to see more details

Western blot analysis of Mouse IL-17A using rat anti mouse IL-17A monoclonal Antibody (Catalog # M00421) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002). Band indicates correct 23 kDa molecular weight position expected for Mouse IL-17A.

Gene/Protein Information For IL17A (Source: Uniprot.org, NCBI)

Alternative Names

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A IL17A CTLA-8, CTLA8, IL-17, IL-17A, IL17 interleukin 17A interleukin-17A|cytotoxic T-lymphocyte-associated antigen 8|cytotoxic T-lymphocyte-associated protein 8|interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)

For more info on IL17A, check out the IL17A Infographic

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In this infographic, you will see the following information for IL17A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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