Anti-MR1 Antibody

Product Name

Anti-MR1 Antibody

View all MR1 Antibodies

SKU/Catalog Number

A00618-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MR1 Antibody catalog # A00618-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00618-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MR1 recombinant protein (Position: R23-D269).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00618-1 is reactive to MR1 in Human, Mouse, Rat

Applications

A00618-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

40 kDa

Calculated molecular weight

39.366kDa

Background of MR1

Major histocompatibility complex class I-related gene protein is a protein that in humans is encoded by the MR1 gene. MAIT (mucosal-associated invariant T-cells) lymphocytes represent a small population of T-cells primarily found in the gut. The protein encoded by this gene is an antigen-presenting molecule that presents metabolites of microbial vitamin B to MAITs. This presentation may activate the MAITs to regulate the amounts of specific types of bacteria in the gut. Several transcript variants encoding different isoforms have been found for this gene, and a pseudogene of it has been detected about 36 kbp upstream on the same chromosome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Flow Cytometry, 1-3μg/1x10⁶ cells
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of MR1 using anti-MR1 antibody (A00618-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human T-47D whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human A431 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MR1 antigen affinity purified polyclonal antibody (Catalog # A00618-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MR1 at approximately 40KD. The expected band size for MR1 is at 43KD.

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Figure 2. Flow Cytometry analysis of SiHa cells using anti-MR1 antibody (A00618-1).
Overlay histogram showing SiHa cells stained with A00618-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MR1 Antibody (A00618-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For MR1 (Source: Uniprot.org, NCBI)

Alternative Names

Class I histocompatibility antigen-like protein; FLJ31593; H2ls; HLALS; major histocompatibility complex class I-related gene protein; major histocompatibility complex, class I-like sequence; major histocompatibility complex, class I-related; MHC class I-like antigen MR-1; MHC class I-related gene protein; MHC class-I related-gene protein; MR1 MR1 HLALS major histocompatibility complex, class I-related major histocompatibility complex class I-related gene protein|MHC class I-like antigen MR-1|MHC class-I related-gene protein|major histocompatibility complex, class I-like sequence

For more info on MR1, check out the MR1 Infographic

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In this infographic, you will see the following information for MR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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