Anti-MRPS30 Antibody

MRPS30 antibody

Boster Bio Anti-MRPS30 Antibody (Catalog # A10227). Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A10227
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-MRPS30 Antibody

View all MRPS30 Antibodies

SKU/Catalog Number

A10227

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MRPS30 Antibody (Catalog # A10227). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MRPS30 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10227)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from C-terminal of human MRPS30.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10227 is reactive to MRPS30 in Human

Applications

A10227 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

50.365kDa

Background of MRPS30

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that is similar to the chicken pro-apoptotic protein p52. Transcript variants using alternative promoters or polyA sites have been mentioned in the literature but the complete description of these sequences is not available.

Carim-Todd L., Cytogenet. Cell Genet. 87:85-88(1999).
Wiemann S., Genome Res. 11-422-435(2001).
Ota T., Nat. Genet. 36:40-45(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For MRPS30 (Source: Uniprot.org, NCBI)

Gene Name

MRPS30

Full Name

39S ribosomal protein S30, mitochondrial

Weight

50.365kDa

Superfamily

mitochondrion-specific ribosomal protein mL65 family

Alternative Names

28S ribosomal protein S30, mitochondrial; DKFZp566B2024; mitochondrial ribosomal protein S30; MRP-S30; PAP; PDCD9; programmed cell death 9; Programmed cell death protein 9; S30mt MRPS30 MRP-S30, PAP, PDCD9, S30mt mitochondrial ribosomal protein S30 39S ribosomal protein S30, mitochondrial|28S ribosomal protein S30, mitochondrial|mitochondrial large ribosomal subunit protein mL65|mitochondrial large ribosomal subunit protein mS30|programmed cell death 9

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MRPS30, check out the MRPS30 Infographic

MRPS30 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MRPS30: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-MRPS30 Antibody

Question

I was wanting to use your anti-MRPS30 antibody for WB for human teratocarcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human teratocarcinoma identification?

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

As indicated on the product datasheet, A10227 anti-MRPS30 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human teratocarcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-14

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for teratocarcinoma using anti-MRPS30 antibody A10227. Let me know if you need anything else.

S. Edwards

Verified customer

Asked: 2020-03-19

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-19

Question

Is a blocking peptide available for product anti-MRPS30 antibody (A10227)?

Verified Customer

Verified customer

Asked: 2018-06-22

Answer

We do provide the blocking peptide for product anti-MRPS30 antibody (A10227). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-06-22

Question

We are currently using anti-MRPS30 antibody A10227 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2018-05-23

Answer

The anti-MRPS30 antibody (A10227) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-05-23

Question

Would anti-MRPS30 antibody A10227 work on feline WB with bone marrow?

Verified Customer

Verified customer

Asked: 2017-12-12

Answer

Our lab technicians have not tested anti-MRPS30 antibody A10227 on feline. You can run a BLAST between feline and the immunogen sequence of anti-MRPS30 antibody A10227 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline bone marrow in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-12-12

Question

Is this A10227 anti-MRPS30 antibody reactive to the isotypes of MRPS30?

B. Dhar

Verified customer

Asked: 2014-11-18

Answer

The immunogen of A10227 anti-MRPS30 antibody is Synthesized peptide derived from C-terminal of human MRPS30. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-11-18

Question

Does anti-MRPS30 antibody A10227 work for WB with teratocarcinoma?

N. Mangal

Verified customer

Asked: 2013-02-14

Answer

According to the expression profile of teratocarcinoma, MRPS30 is highly expressed in teratocarcinoma. So, it is likely that anti-MRPS30 antibody A10227 will work for WB with teratocarcinoma.

Boster Scientific Support

Answered: 2013-02-14

Order DetailsPrice
A10227-100uL

100uL

$360

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A10227
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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