Anti-MT2-MMP/MMP15 Antibody Picoband™

Product Name

Anti-MT2-MMP/MMP15 Antibody Picoband™

View all MMP-15/MT2-MMP Antibodies

SKU/Catalog Number

A06396-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MT2-MMP/MMP15 Antibody Picoband™ catalog # A06396-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MT2-MMP/MMP15 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06396-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MT2-MMP/MMP15 recombinant protein (Position: Y132-A202).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A06396-1 is reactive to MMP15 in Human, Mouse, Rat

Applications

A06396-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

74 kDa

Calculated molecular weight

75.807kDa

Background of MMP-15/MT2-MMP

Matrix metalloproteinase 15 also known as MMP15 is an enzyme that in humans is encoded by the MMP15 gene. This gene encodes a member of the peptidase M10 family and membrane-type subfamily of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Members of this subfamily contain a transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. The encoded preproprotein is proteolytically processed to generate the mature protease. This protein may play a role in cancer progression.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of MT2-MMP/MMP15 using anti-MT2-MMP/MMP15 antibody (A06396-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: rat RH35 whole cell lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MT2-MMP/MMP15 antigen affinity purified polyclonal antibody (Catalog # A06396-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MT2-MMP/MMP15 at approximately 74KD. The expected band size for MT2-MMP/MMP15 is at 74KD.

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Figure 2. IF analysis of MT2-MMP/MMP15 using anti-MT2-MMP/MMP15 antibody (A06396-1).
MT2-MMP/MMP15 was detected in immunocytochemical section of HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-MT2-MMP/MMP15 Antibody (A06396-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-MT2-MMP/MMP15 antibody (A06396-1).
Overlay histogram showing CACO-2 cells stained with A06396-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MT2-MMP/MMP15 Antibody (A06396-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For MMP15 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.4.24; EC 3.4.24.-; EC 3.4.24.80; matrix metallopeptidase 15 (membrane-inserted); matrix metalloproteinase 15 (membrane-inserted); Membrane-type matrix metalloproteinase 2; Membrane-type-2 matrix metalloproteinase; MMP15; MMP-15; MT2-MMP; MT2-MMPMT2MMP; MTMMP2MT-MMP 2; SMCP-2matrix metalloproteinase-15 MMP15 MMP-15, MT2-MMP, MT2MMP, MTMMP2, SMCP-2 matrix metallopeptidase 15 matrix metalloproteinase-15|MT-MMP 2|matrix metallopeptidase 15 (membrane-inserted)|matrix metalloproteinase 15 (membrane-inserted)|membrane-type matrix metalloproteinase 2|membrane-type-2 matrix metalloproteinase

For more info on MMP15, check out the MMP15 Infographic

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In this infographic, you will see the following information for MMP15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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