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Product Info Summary
| SKU: | PA1660 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-MyD88 Antibody
SKU/Catalog Number
PA1660
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MyD88 Antibody catalog # PA1660. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MyD88 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1660)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human MyD88, different from the related rat and mouse sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1660 is reactive to MYD88 in Human, Mouse, Rat
Applications
PA1660 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
19 kDa
Calculated molecular weight
33.233kDa
Background of MYD88
MYD88 (MYELOID DIFFERENTIATION PRIMARY RESPONSE GENE 88), is a protein that, in humans, is encoded by the MYD88 gene. MyD88 is a key downstream adapter for most Toll-like receptors (TLRs) and interleukin-1 receptors (IL1Rs). And it is mapped on 3p22.2. MYD88 encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. Qverexpression of MYD88 caused an increase in the level of transcription from the interleukin-8 promoter. The C-terminal domain of MYD88 has significant sequence similarity to the cytoplasmic domain of IL1RAP. Inhibiting the IL1R-MYD88 pathway in vivo could block the damage from acute inflammation that occurs in response to sterile cell death, and do so in a way that might not compromise tissue repair or host defense against pathogens.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-MyD88 antibody, PA1660, Western blotting
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: RAJI Cell Lysate
Click image to see more details
Anti-MyD88 antibody, PA1660, IHC(P)
IHC(P): Human Tonsil Tissue
Protein Target Info & Infographic
Gene/Protein Information For MYD88 (Source: Uniprot.org, NCBI)
Gene Name
MYD88
Full Name
Myeloid differentiation primary response protein MyD88
Weight
33.233kDa
Alternative Names
MyD88; MYD88D; myeloid differentiation primary response gene (88); myeloid differentiation primary response protein MyD88 MYD88 IMD68D, MYD88 MYD88 innate immune signal transduction adaptor myeloid differentiation primary response protein MyD88|mutant myeloid differentiation primary response 88|myeloid differentiation primary response 88|myeloid differentiation primary response gene (88)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYD88, check out the MYD88 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYD88: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MyD88 Antibody (PA1660)
Hello CJ!
PA1660 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li X,Shi MQ,Chen C,Du JR.Phthalide derivative CD21 ameliorates ischemic brain injury in a mouse model of global cerebral ischemia: involvement of inhibition of NLRP3.Int Immunopharmacol.2020 Sep;86:106714.doi: 10.1016/j.intimp.2020.106714.Epub 2020 Jun 24
Species: Mouse
PA1660 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:250
Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response
Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling pathway
High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy
Toll-Like Receptor 4 Prompts Human Breast Cancer Cells Invasiveness via Lipopolysaccharide Stimulation and Is Overexpressed in Patients with Lymph Node Metastasis
Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
17 Customer Q&As for Anti-MyD88 Antibody
Question
Do you have a BSA free version of anti-MyD88 antibody PA1660 available?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MyD88 antibody PA1660 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-21
Question
We are currently using anti-MyD88 antibody PA1660 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
The anti-MyD88 antibody (PA1660) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-13
Question
My colleagues were satisfied with the WB result of your anti-MyD88 antibody. However we have observed positive staining in pancreas cytoplasm using this antibody. Is that expected? Could you tell me where is MYD88 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
From what I have seen in literature, pancreas does express MYD88. Generally MYD88 expresses in cytoplasm. Regarding which tissues have MYD88 expression, here are a few articles citing expression in various tissues:
Dendritic cell, Pubmed ID: 8957090
Epidermal carcinoma, Pubmed ID: 9013863
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334
Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-16
Question
Does anti-MyD88 antibody PA1660 work for WB with erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
According to the expression profile of erythroleukemia, MYD88 is highly expressed in erythroleukemia. So, it is likely that anti-MyD88 antibody PA1660 will work for WB with erythroleukemia.
Boster Scientific Support
Answered: 2019-09-25
Question
We need using your anti-MyD88 antibody for 3'-utr-mediated mrna stabilization studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
Thank you for your inquiry. This PA1660 anti-MyD88 antibody is tested on rat thymus tissue, spleen tissue, tissue lysate, jurkat cell lysate, raji cell lysate, human tonsil tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-05
Question
Would PA1660 anti-MyD88 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Anderson
Verified customer
Asked: 2019-01-11
Answer
It shows on the product datasheet, PA1660 anti-MyD88 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-11
Question
My question regarding product PA1660, anti-MyD88 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1660 anti-MyD88 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-07
Question
I was wanting to use your anti-MyD88 antibody for WB for rat erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2018-03-06
Answer
As indicated on the product datasheet, PA1660 anti-MyD88 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-06
Question
We ordered your anti-MyD88 antibody for WB on blood in the past. I am using mouse, and We want to use the antibody for IHC next. My lab would like examining blood as well as epidermal carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-02-14
Answer
I looked at the website and datasheets of our anti-MyD88 antibody and it seems that PA1660 has been validated on mouse in both WB and IHC. Thus PA1660 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-02-14
Question
I would like to test anti-MyD88 antibody PA1660 on rat erythroleukemia for research purposes, then I may be interested in using anti-MyD88 antibody PA1660 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-30
Answer
The products we sell, including anti-MyD88 antibody PA1660, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-30
Question
We have been able to see staining in human blood. Do you have any suggestions? Is anti-MyD88 antibody supposed to stain blood positively?
A. Johnson
Verified customer
Asked: 2017-09-04
Answer
According to literature blood does express MYD88. According to Uniprot.org, MYD88 is expressed in blood, dendritic cell, epidermal carcinoma, umbilical cord blood, pancreas, erythroleukemia, among other tissues. Regarding which tissues have MYD88 expression, here are a few articles citing expression in various tissues:
Dendritic cell, Pubmed ID: 8957090
Epidermal carcinoma, Pubmed ID: 9013863
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334
Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-09-04
Question
Does anti-MyD88 antibody PA1660 work on canine IHC with epidermal carcinoma?
N. Li
Verified customer
Asked: 2016-12-20
Answer
Our lab technicians have not validated anti-MyD88 antibody PA1660 on canine. You can run a BLAST between canine and the immunogen sequence of anti-MyD88 antibody PA1660 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine epidermal carcinoma in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-12-20
Question
See below the WB image, lot number and protocol we used for erythroleukemia using anti-MyD88 antibody PA1660. Please let me know if you require anything else.
T. Jha
Verified customer
Asked: 2016-04-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-04-13
Question
Is a blocking peptide available for product anti-MyD88 antibody (PA1660)?
Z. Mitchell
Verified customer
Asked: 2015-12-11
Answer
We do provide the blocking peptide for product anti-MyD88 antibody (PA1660). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-12-11
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-MyD88 antibody PA1660. Let me know if you need anything else.
N. Johnson
Verified customer
Asked: 2014-05-19
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-05-19
Question
I see that the anti-MyD88 antibody PA1660 works with WB, what is the protocol used to produce the result images on the product page?
E. Evans
Verified customer
Asked: 2013-05-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-05-01
Question
Is this PA1660 anti-MyD88 antibody reactive to the isotypes of MYD88?
O. Walker
Verified customer
Asked: 2013-02-25
Answer
The immunogen of PA1660 anti-MyD88 antibody is A synthetic peptide corresponding to a sequence in the middle region of human MyD88(174-188aa FVQEMIRQLEQTNYR), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-02-25
