Product Info Summary
SKU: | CI1028 |
---|---|
Size: | 100μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ChIP, ChIP-seq, ELISA, WB |
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Product info
Product Name
Anti-Myosin-11 MYH11 Antibody
View all Myosin heavy chain 11 Antibodies
SKU/Catalog Number
CI1028
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Myosin-11 MYH11 Antibody (Catalog# CI1028). Tested in ChIP, ChIP-seq, ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C. For long-term storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Cite This Product
Anti-Myosin-11 MYH11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # CI1028)
Host
Rabbit
Contents
Whole antiserum from rabbit containing 0.05% azide.
Clonality
Polyclonal
Immunogen
This antibody is raised in rabbit against human MYH11 (Myosin, Heavy Chain 11) using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
CI1028 is reactive to MYH11 in Human
Applications
CI1028 is guaranteed for ChIP, ChIP-seq, ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
227.339kDa
Background of Myosin heavy chain 11
MYH11 is a smooth muscle myosin belonging to the myosin heavy chain family which function as major contractile proteins. MYH11 is involved in a pericentric inversion of chromosome 16 (inv (16) (p13q22)) which produces a chimeric transcript consisting of the N terminus of CBFb and the C-terminal portion MYH11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype.References citing this antibody (1) Mandoli A, Singh AA, Jansen PWTC, Wierenga ATJ, Riahi H, Franci G, Prange K, Saeed S, Vellenga E, Vermeulen M, Stunnenberg HG and Martens JHA (2013) CBFB–MYH11/RUNX1 together with a compendium of hematopoietic regulators, chromatin modifiers and basal transcription factors occupies self-renewal genes in inv (16) acute myeloid leukemia. Leukemia: 1-9.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
ChIP assays were performed using ME-1 cells, Anti-MYH11 polyclonal antibody (Catalog # CI1028) and optimized primer pairs for qPCR. QPCR was performed using primers specific for the genes indicated.
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ChIP was performed with 5 μl of Anti-MYH11 polyclonal antibody (Catalog # CI1028)on sheared chromatin from 1.5 million cells. The IP DNA from 6 ChIP was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.
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To determine the titer of the antibody, an ELISA was performed using a serial dilution of Anti-MYH11 polyclonal antibody (Catalog # CI1028). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1,900.
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Western blot analysis of MYH11 expression in nuclear extracts from ME-1 cells. CBFb was detected using Anti-CBFb antibody, whereas CBFb-MYH11 fusion proteins were detected using Anti-CBFb antibody and MYH11 polyclonal antibody (Catalog # CI1028) at 1/1000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For MYH11 (Source: Uniprot.org, NCBI)
Gene Name
MYH11
Full Name
Myosin-11
Weight
227.339kDa
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
AAT4; FAA4; MYH11 myosin, heavy chain 11, smooth muscle; MYH11; SMHC; SMMHC; Smooth muscle myosin heavy chain MYH11 AAT4, FAA4, SMHC, SMMHC myosin heavy chain 11 myosin-11|epididymis secretory sperm binding protein|myosin, heavy chain 11, smooth muscle|myosin, heavy polypeptide 11, smooth muscle
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYH11, check out the MYH11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYH11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myosin-11 MYH11 Antibody (CI1028)
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No publications found for CI1028
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