Anti-Myosin-6 MYO6 Antibody

Product Name

Anti-Myosin-6 MYO6 Antibody

View all MYO6 Antibodies

SKU/Catalog Number

A02627

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Myosin-6 MYO6 Antibody (Catalog # A02627). Tested in WB applications. This antibody reacts with Human, Pig.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Myosin-6 MYO6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02627)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

Anti-Myosin-6 was prepared from whole rabbit serum produced by repeated immunizations with full length myosin-6 construct expressed in SF9 insect cells corresponding to porcine myosyn-6 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02627 is reactive to MYO6 in Human, Pig

Applications

A02627 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

149.691kDa

Background of MYO6

Myosin VI is a myosin superfamily member with unique and intriguing features that allow it to fill a still-expanding number of cell biological roles. This actin-based motor produces force that acts towards the minus end of actin filaments, which is the opposite direction to all other characterized myosins. In mammalian cells, myosin VI is localized to endocytic vesicles, membrane ruffles, the cytosol and the Golgi complex. Its motor function is essential for several physiological functions of the cell, including normal rates of endocytosis, maintenance of Golgi morphology and protein secretion. Myosin VI regulates epithelial cell migration and plays a role in the maintenance of adhesive cellular contacts within epithelial cell layers. It is highly expressed in ovarian cancers and prostate cancers and its expression level, which is upregulated by DNA damage in a p53-dependent manner, correlates with the potential of the tumor to disseminate. More recently, myosin VI has been found involved in EGFR endocytosis through a clathrin dependent mechanism.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000
WB: 1:1000

Validation Images & Assay Conditions

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Western blot analysis of Myosin-6 expression in SF9 cell lysates of truncated smooth myosin (lane 1), Jurkat whole cell lysates (lane 2), LnCap whole cell lysates (lane 3) and recombinant myosin-6 protein (lane 4). Myosin-6 at 150KD was detected using rabbit anti-Myosin-6 not purified polyclonal antibody (Catalog # A02627) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For MYO6 (Source: Uniprot.org, NCBI)

Alternative Names

DFNA22; DFNB37; KIAA0389deafness, autosomal recessive 37; myosin VI; myosin-VI; Unconventional myosin-6 MYO6 DFNA22, DFNB37 myosin VI unconventional myosin-VI|unconventional myosin-6

For more info on MYO6, check out the MYO6 Infographic

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In this infographic, you will see the following information for MYO6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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