Anti-NEK2 Antibody

Product Name

Anti-NEK2 Antibody

View all NEK2 Antibodies

SKU/Catalog Number

A01606

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-NEK2 Antibody (Catalog # A01606). Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-NEK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01606)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Clone Number

CFA-18

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 275-300 of Human NEK2 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Predicted species reactivity based on epitope sequence homology: human, mouse, bovine, horse, rhesus macaque, dog, chimpanzee.

Reactive Species

A01606 is reactive to NEK2 in Mouse

Applications

A01606 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

51.763kDa

Background of NEK2

NEK2, also called serine/threonine-protein kinase Nek2NimA-related protein kinase 2, NimA-like protein kinase 1 and HSPK 21, is a protein kinase involved in mitotic regulation that may have a role at the G₂-M transition and may also play a role in meiosis. NEK2 has a nuclear localization and accumulates throughout S phase and shows maximal levels in late G₂. This expression pattern is highly reminiscent of that of A and B cyclins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IF Microscopy: User optimized
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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Western blot analysis of NEK2 expression in A-20 whole cell lysates (lane 3). NEK2 at 52KD was detected using rabbit anti-NEK2 Antigen Affinity purified polyclonal antibody (Catalog # A01606) at 1:500. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.

Gene/Protein Information For NEK2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.11; EC 2.7.11.1; HSPK 21; HsPK21; NEK2; NEK2AHsPK21; Never in mitosis A-related kinase 2; NIMA (never in mitosis gene a)-related kinase 2; NimA-like protein kinase 1; NimA-related protein kinase 2; NLK1; NLK1serine/threonine-protein kinase Nek2 NEK2 HsPK21A, NLK1, PPP1R111, RP67, NEK2 NIMA related kinase 2 serine/threonine-protein kinase Nek2|NIMA (never in mitosis gene a)-related kinase 2|nimA-like protein kinase 1|nimA-related protein kinase 2|protein phosphatase 1, regulatory subunit 111

For more info on NEK2, check out the NEK2 Infographic

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In this infographic, you will see the following information for NEK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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