Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1)

Product Name

Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1)

View all nNOS Antibodies

SKU/Catalog Number

MA1072

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Nitric Oxide Synthase, Brain (1-181) NOS1 Antibody (Monoclonal, NOS-B1) catalog # MA1072. Tested in WB applications. This antibody reacts with Goat, Human, Pig, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1072)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

NOS-B1

Isotype

Mouse IgG1

Immunogen

Recombinant neuronal NOS fragment (amino acids 1-181) from rat brain.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1072 is reactive to NOS1 in Goat, Human, Pig, Rat

Applications

MA1072 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

200 kDa

Calculated molecular weight

160.97kDa

Background of nNOS

Nitric Oxide Synthase 1 (NOS1,neuronal NOS,nNOS1) is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. It may be involved in neuronal cell death and damage in neurological illness. nNOS1 localized to the 12q24.2 region of human chromosome 12. It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098 amino acids. nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2. Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length. The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle. The novel gaseous neuromediator nitric oxide is though to play an important role in development and plasticity. Despite this, gene-knockout mice lacking neuronal (Type I) nitric oxide synthase exhibit relatively normal brain development and behavior.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.5μg/ml, Human, goat, pig, rat

Validation Images & Assay Conditions

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Boster Kit Box

Gene/Protein Information For NOS1 (Source: Uniprot.org, NCBI)

Alternative Names

bNOS; brain; Constitutive NOS; EC 1.14.13.39; IHPS1; Neuronal NOS; nitric oxide synthase 1 (neuronal); nNOS; N-NOS; nNOSNOS type I; NOS; NOS1; Peptidyl-cysteine S-nitrosylase NOS1 NOS1 IHPS1, N-NOS, NC-NOS, NOS, bNOS, nNOS nitric oxide synthase 1 nitric oxide synthase, brain|NOS type I|constitutive NOS|neuronal NOS|nitric oxide synthase 1 (neuronal)|peptidyl-cysteine S-nitrosylase NOS1

For more info on NOS1, check out the NOS1 Infographic

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In this infographic, you will see the following information for NOS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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