Anti-Nitric oxide synthase (inducible) Nos2 Antibody

Product Name

Anti-Nitric oxide synthase (inducible) Nos2 Antibody

View all NOS2 Antibodies

SKU/Catalog Number

A00368-1

Size

100µl

Form

Liquid

Description

Boster Bio Anti-Nitric oxide synthase (inducible) Nos2 Antibody (Catalog# A00368-1). Tested in ICC, IHC, WB application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -80°C for long-term storage. Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Cite This Product

Anti-Nitric oxide synthase (inducible) Nos2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00368-1)

Host

Rabbit

Contents

Liquid. In PBS containing 0.05% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic peptide corresponding to aa 1131-1141 of mouse macrophage NOS

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with eNOS or nNOS.

Reactive Species

A00368-1 is reactive to NOS2 in Human, Mouse, Rat

Applications

A00368-1 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

131.117kDa

Background of NOS2

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

David A. Jans, J. Biol. Chem., Nov 1996; 271: 30781.
Eugene Estella, Diabetes, Aug 2006; 55: 2212 - 2219.
Yong Gu, J. Biol. Chem., May 1996; 271: 10816.
Jiuru Sun, J. Biol. Chem., Nov 1996; 271: 27802.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry (1:500)
Immunohistochemistry (1:500)
Western Blot (1:2,000). Detects a band of ~130kDa.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Nos2 using anti-Nos2 antibody (A00368-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nos2 antigen affinity purified polyclonal antibody (Catalog # A00368-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Nos2.

Gene/Protein Information For NOS2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 1.14.13.39; Hepatocyte NOS; HEP-NOSPeptidyl-cysteine S-nitrosylase NOS2; Inducible NO synthase; Inducible NOS; iNOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, inducible; nitric oxide synthase, macrophage; NOS; NOS, type II; NOS2; NOS2A; NOS2ANOS type II NOS2 HEP-NOS, INOS, NOSA, NOS2 nitric oxide synthase 2 nitric oxide synthase, inducible|NOS, type II|hepatocyte NOS|inducible NO synthase|inducible NOS|nitric oxide synthase 2, inducible|nitric oxide synthase 2A (inducible, hepatocytes)|nitric oxide synthase, macrophage|peptidyl-cysteine S-nitrosylase NOS2

For more info on NOS2, check out the NOS2 Infographic

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In this infographic, you will see the following information for NOS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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