Anti-Nitric oxide synthase (inducible) clonal Monoclonal Antibody (K13-A)

Product Name

Anti-Nitric oxide synthase (inducible) clonal Monoclonal Antibody (K13-A)

View all NOS2 Antibodies

SKU/Catalog Number

M00368-1

Size

100µl

Form

Liquid

Description

Boster Bio Anti-Nitric oxide synthase (inducible) clonal Monoclonal Antibody (K13-A) (Catalog# M00368-1). Tested in ELISA, IHC-F, IHC-P, IP, WB application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Nitric oxide synthase (inducible) clonal Monoclonal Antibody (K13-A) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00368-1)

Host

Rabbit

Contents

Liquid. In 20mM TRIS/HCl, pH 8.0, containing 10mg/ml BSA and 0.05% sodium azide.

Clonality

Monoclonal

Clone Number

K13-A

Isotype

IgG

Immunogen

Synthetic peptide corresponding to a portion of human inducible nitric oxide synthase (iNOS; NOS II)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00368-1 is reactive to NOS2 in Human, Mouse, Rat

Applications

M00368-1 is guaranteed for ELISA, IP, IHC-P, IHC-F, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

131.117kDa

Background of NOS2

Paired box protein Pax-6 also known as aniridia type II protein (AN2) or oculorhombin is a transcription factor with key functions in the development of the eye, nose, central nervous system and pancreas. Pax6 further seems to be required for the differentiation of pancreatic islet alpha cells. PAX6 has been shown to bind to a promoter element in the glucagon, insulin and somatostatin promoters and regulates the specification of the ventral neuron subtypes by establishing the correct progenitor domains. Isoform 5a appears to function as a molecular switch that specifies target genes. Anti-PAX6 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA (1:20,000-1:50,000)
Western Blot (1:2,000; Wash buffer: 1x TRIS buffered saline (TBS) containing 0.1% Triton X-100, Blocking buffer: 1x TBS containing 0.1% Triton X-100 and 5% nonfat dry milk. Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature)
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Figure 1. IHC analysis of NOS2 using anti-NOS2 antibody (M00368-1).
NOS2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NOS2 Antibody (M00368-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For NOS2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 1.14.13.39; Hepatocyte NOS; HEP-NOSPeptidyl-cysteine S-nitrosylase NOS2; Inducible NO synthase; Inducible NOS; iNOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, inducible; nitric oxide synthase, macrophage; NOS; NOS, type II; NOS2; NOS2A; NOS2ANOS type II NOS2 HEP-NOS, INOS, NOSA, NOS2 nitric oxide synthase 2 nitric oxide synthase, inducible|NOS, type II|hepatocyte NOS|inducible NO synthase|inducible NOS|nitric oxide synthase 2, inducible|nitric oxide synthase 2A (inducible, hepatocytes)|nitric oxide synthase, macrophage|peptidyl-cysteine S-nitrosylase NOS2

For more info on NOS2, check out the NOS2 Infographic

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In this infographic, you will see the following information for NOS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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