Product Info Summary
SKU: | A00634 |
---|---|
Size: | 200uL |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Osteopontin SPP1 Antibody
View all Osteopontin/OPN Antibodies
SKU/Catalog Number
A00634
Size
200uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Osteopontin SPP1 Antibody (Catalog # A00634). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-Osteopontin SPP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00634)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, from the human osteopontin protein, conjugated to KLH using maleimide.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00634 is reactive to SPP1 in Human
Applications
A00634 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
35.423kDa
Background of Osteopontin/OPN
Anti-Osteopontin Antibody recognizes Osteopontin (OPN) which is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. OPN is multifunctional, with activities in cell migration, cell survival, inhibition of calcification, regulation of immune cell function, and control of tumor cell phenotype. The gene encoding OPN is called spp1. Targeting this gene has revealed that while OPN is not necessary for normal embryonic development, fertility, and health under pathogen-free conditions, loss of the protein has significant consequences in several models of injury/disease as diverse as renal injury, viral, and bacterial infection, bone remodeling, and tumor growth. The fact that no other proteins seem to share a redundant activity with OPN under these conditions suggests that OPN has a unique functional role during tissue injury and stress. Interestingly, several members of the matrix metalloproteinase (MMP) family are also induced during injury/disease processes in patterns overlapping that of OPN. OPN has recently been shown to be a novel substrate for two MMPs, MMP-3 (stromelysin-1) and MMP-7 (matrilysin). There are three cleavage sites for MMP-3 in human OPN, two of which are also cleaved by MMP-7 (see cleavage diagram). Biological assays demonstrate that the MMP-cleaved OPN has increased activity in promoting both cell adhesion and migration compared with full-length OPN. In addition, inhibitory reagents were used to show that the same receptors that interact with OPN also mediate interaction of MMP-cleaved OPN with tumor cells. It is suggested that active forms of OPN at sites of tissue injury may be regulated by the activity of proteases including MMPs and that the differences in activity of modified OPN may be explained by differences in binding affinity of integrins or distinct downstream signaling events. Osteopontin can be responsible for diseases such as lung and prostate cancers, nephrolithiasis, hepatocellular carcinoma, osteoporosis and arteriosclerosis. Anti-Osteopontin is useful for researchers interested in Stem Cell and Extracellular Matrix Antibodies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IHC: 1:100 - 1:300
IP: 1:100
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
Click image to see more details
OPN is cleaved by MMP to yield 2 fragments, which migrate at 40 kDa (N terminal) and 32 kDa (C terminal). The C terminal fragment can undergo further cleavage by both of these MMPs. The epitope that is recognized by Rabbit-antiOsteopontin is shown in violet. This antibody detects the full length OPN and the 32 kDa fragment. It does not recognize the 40 kDa fragment.
Click image to see more details
Rabbit anti-Osteopontin was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry.Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.
Click image to see more details
Western Blot of Rabbit Anti-Osteopontin Antibody. Lane 1: Molecular Weight Marker. Lane 2: Human Osteopontin [250ng]. Lane 3: MMP-cleaved Osteopontin. Primary Antibody: Anti-Osteopontin at 1:1000 ovenight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:10,000. Observed MW: full length 66kDa, MMPcleaved 32kDa
Protein Target Info & Infographic
Gene/Protein Information For SPP1 (Source: Uniprot.org, NCBI)
Gene Name
SPP1
Full Name
Osteopontin
Weight
35.423kDa
Superfamily
osteopontin family
Alternative Names
BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin SPP1 BNSP, BSPI, ETA-1, OPN secreted phosphoprotein 1 osteopontin|SPP1/CALPHA1 fusion|early T-lymphocyte activation 1|nephropontin|osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein|secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)|secreted phosphoprotein 1 variant 6|urinary stone protein|uropontin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPP1, check out the SPP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Osteopontin SPP1 Antibody (A00634)
Hello CJ!
A00634 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Traditional Chinese medicine formula Bi-Qi capsule alleviates rheumatoid arthritis-induced inflammation, synovial hyperplasia, and cartilage destruction in rats
Avβ3 single‐stranded DNA aptamer attenuates vascular restenosis via Ras‐PI3K/MAPK pathway in rats after percutaneous transluminal coronary angioplasty
An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties
Functionalizing titanium surface with PAMAM dendrimer and human BMP2 gene via layer‐by‐layer assembly for enhanced osteogenesis
Caiyun Mu,Ye He,Yan Hu,Menghuan Li,Maowen Chen,Rong Wang,Yang Xiang, Zhong Luo,Kaiyong Cai,Construction of chemokine substance P-embedded biomimetic multilayer onto bioactive magnesium silicate-titanium implant for bone regeneration,Applied Materials Toda
Species: Mouse,Rat
A00634 usage in article: APP:WB, SAMPLE:MSCS, DILUTION:NA
Chaowei Hu,Kun Zuo,Kuibao Li,Yuanfeng Gao,Mulei Chen,Roumu Hu,Ye Liu,Hongjie Chi,Hongjiang Wang,Yanwen Qin,Xiaoyan Liu,Jiuchang Zhong,Jun Cai,Xinchun Yang,Jing Li, "p38/JNK Is Required for the Proliferation and Phenotype Changes of Vascular Smooth Muscle
Species: Rat,Human
A00634 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION:NA
Paracrine effect of inflammatory cytokine-activated bone marrow mesenchymal stem cells and its role in osteoblast function
Traditional Chinese medicine formula Bi-Qi capsule alleviates rheumatoid arthritis-induced inflammation, synovial hyperplasia, and cartilage destruction in %u2026
The Osteogenic Potential of Mesoporous Bioglasses/Silk and Non-Mesoporous Bioglasses/Silk Scaffolds in Ovariectomized Rats: In vitro and In vivo Evaluation
Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated with Activation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Osteopontin SPP1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Osteopontin SPP1 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
15 Customer Q&As for Anti-Osteopontin SPP1 Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for milk using anti-Osteopontin antibody A00634. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-23
Question
Is a blocking peptide available for product anti-Osteopontin antibody (A00634)?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
We do provide the blocking peptide for product anti-Osteopontin antibody (A00634). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-15
Question
We have observed staining in human pituitary. What should we do? Is anti-Osteopontin antibody supposed to stain pituitary positively?
D. Krishna
Verified customer
Asked: 2019-11-08
Answer
Based on literature pituitary does express SPP1. Based on Uniprot.org, SPP1 is expressed in amniotic fluid, liver, brain, kidney subthalamic nucleus, placenta, brain kidney, kidney, milk, pituitary, among other tissues. Regarding which tissues have SPP1 expression, here are a few articles citing expression in various tissues:
Brain, and Kidney, Pubmed ID: 15489334
Kidney, Pubmed ID: 1575754
Kidney, and Subthalamic nucleus, Pubmed ID: 14702039
Liver, Pubmed ID: 7945249, 24275569
Milk, Pubmed ID: 2736258, 15869464
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 16303743
Boster Scientific Support
Answered: 2019-11-08
Question
Does A00634 anti-Osteopontin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
As indicated on the product datasheet, A00634 anti-Osteopontin antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-07
Question
I have a question about product A00634, anti-Osteopontin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00634 anti-Osteopontin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-07
Question
I am interested in to test anti-Osteopontin antibody A00634 on human milk for research purposes, then I may be interested in using anti-Osteopontin antibody A00634 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The products we sell, including anti-Osteopontin antibody A00634, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-27
Question
Does anti-Osteopontin antibody A00634 work for WB with milk?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
According to the expression profile of milk, SPP1 is highly expressed in milk. So, it is likely that anti-Osteopontin antibody A00634 will work for WB with milk.
Boster Scientific Support
Answered: 2019-08-14
Question
I see that the anti-Osteopontin antibody A00634 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-31
Question
We were well pleased with the WB result of your anti-Osteopontin antibody. However we have seen positive staining in pituitary secreted. using this antibody. Is that expected? Could you tell me where is SPP1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
According to literature, pituitary does express SPP1. Generally SPP1 expresses in secreted. Regarding which tissues have SPP1 expression, here are a few articles citing expression in various tissues:
Brain, and Kidney, Pubmed ID: 15489334
Kidney, Pubmed ID: 1575754
Kidney, and Subthalamic nucleus, Pubmed ID: 14702039
Liver, Pubmed ID: 7945249, 24275569
Milk, Pubmed ID: 2736258, 15869464
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 16303743
Boster Scientific Support
Answered: 2019-06-13
Question
Do you have a BSA free version of anti-Osteopontin antibody A00634 available?
Verified Customer
Verified customer
Asked: 2019-04-22
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Osteopontin antibody A00634 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-22
Question
Is this A00634 anti-Osteopontin antibody reactive to the isotypes of SPP1?
Verified Customer
Verified customer
Asked: 2017-06-27
Answer
The immunogen of A00634 anti-Osteopontin antibody is This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, from the human osteopontin protein, conjugated to KLH using maleimide. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-27
Question
Please see the WB image, lot number and protocol we used for milk using anti-Osteopontin antibody A00634. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-06-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-14
Question
I was wanting to use your anti-Osteopontin antibody for WB for human milk on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human milk identification?
K. Krishna
Verified customer
Asked: 2017-05-11
Answer
You can see on the product datasheet, A00634 anti-Osteopontin antibody has been validated for IP, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human milk in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-11
Question
We are currently using anti-Osteopontin antibody A00634 for mouse tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
J. Jones
Verified customer
Asked: 2013-10-15
Answer
The anti-Osteopontin antibody (A00634) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-10-15
Question
We have tried in the past anti-Osteopontin antibody for IHC on milk a few years ago. I am using human, and We are going to use the antibody for WB next. We need examining milk as well as kidney in our next experiment. Could give a recommendation on which antibody would work the best for WB?
O. Carter
Verified customer
Asked: 2013-01-03
Answer
I viewed the website and datasheets of our anti-Osteopontin antibody and it seems that A00634 has been tested on human in both IHC and WB. Thus A00634 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-01-03