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Product Info Summary
| SKU: | M00173 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-p27Kip1 (Mitotic Inhibitor/Suppressor Protein) CDKN1B Monoclonal Antibody
SKU/Catalog Number
M00173
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-p27Kip1 (Mitotic Inhibitor/Suppressor Protein) CDKN1B Monoclonal Antibody (Catalog # M00173). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-p27Kip1 (Mitotic Inhibitor/Suppressor Protein) CDKN1B Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00173)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: DCS-72.F6 + KIP1/769
Isotype
IgG1, kappa
Immunogen
Mouse recombinant p27 protein (DCS-72.F6); Recombinant human CDKN1B protein (KIP1/769)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00173 is reactive to CDKN1B in Human, Monkey, Mouse, Rat
Applications
M00173 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
22.073kDa
Background of p27/Kip1
Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This monoclonal antibody co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Colon Cancer stained with Anti-p27 Monoclonal Antibody (DCS-72.F6 + KIP1/769)
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SDS-PAGE analysis purified Anti-p27 Monoclonal Antibody (DCS-72.F6 + KIP1/769). Confirmation of purity and integrity of antibody.
Protein Target Info & Infographic
Gene/Protein Information For CDKN1B (Source: Uniprot.org, NCBI)
Gene Name
CDKN1B
Full Name
Cyclin-dependent kinase inhibitor 1B
Weight
22.073kDa
Superfamily
CDI family
Alternative Names
CDKN1B; CDKN4; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Kip1; KIP1P27KIP1; MEN1B; MEN4; p27; p27Kip1 CDKN1B CDKN4, KIP1, MEN1B, MEN4, P27KIP1 cyclin dependent kinase inhibitor 1B cyclin-dependent kinase inhibitor 1B|cyclin-dependent kinase inhibitor 1B (p27, Kip1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDKN1B, check out the CDKN1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDKN1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p27Kip1 (Mitotic Inhibitor/Suppressor Protein) CDKN1B Monoclonal Antibody (M00173)
Hello CJ!
M00173 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Hepatitis B virus induces G1 phase arrest by regulating cell cycle genes in HepG2.2.15 cells
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