Anti-p48-DDB2 Antibody

Product Name

Anti-p48-DDB2 Antibody

View all XPE/DDB2 Antibodies

SKU/Catalog Number

A01430

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-p48-DDB2 Antibody (Catalog # A01430). Tested in IP, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-p48-DDB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01430)

Host

Rabbit

Contents

0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Clone Number

3B5

Isotype

Antiserum

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 419-427 of Human DDB2 (C-terminal) coupled to KLH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01430 is reactive to DDB2 in Human

Applications

A01430 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

47.864kDa

Background of XPE/DDB2

DDB2 is also known as Damage-specific DNA binding protein 2, DDB p48 subunit, DDBb, UV-damaged DNA-binding protein 2 and UV-DDB 2. The DDB2 gene encodes the small subunit (p48) of DNA damage-binding protein, which is a heterodimer, composed of a large (p127 DDB1) and a small subunit. The DDB2 subunit appears to be required for DNA binding. This nuclear protein functions in nucleotide-excision repair resulting from UV-damaged DNA by binding to pyrimidine dimers. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). XP-E is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the large subunit.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IHC: User optimized
IP: User optimized
WB: 1:500 - 1:1,000

Validation Images & Assay Conditions

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Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.Data contributed by S.Jentsch.

Gene/Protein Information For DDB2 (Source: Uniprot.org, NCBI)

Alternative Names

damage-specific DNA binding protein 2 (48kD); damage-specific DNA binding protein 2, 48kDa; Damage-specific DNA-binding protein 2; DDB p48 subunit; DDB2; DDBB; DNA damage-binding protein 2; FLJ34321; UV-damaged DNA-binding protein 2; UV-DDB 2; UV-DDB2; xeroderma pigmentosum group E protein; XP5; XPE/DDB2 DDB2 DDBB, UV-DDB2, XPE damage specific DNA binding protein 2 DNA damage-binding protein 2|DDB p48 subunit|UV-DDB 2|UV-damaged DNA-binding protein 2|damage-specific DNA binding protein 2, 48kDa|xeroderma pigmentosum group E protein

For more info on DDB2, check out the DDB2 Infographic

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In this infographic, you will see the following information for DDB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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