Anti-PARP-1 Antibody

Product Name

Anti-PARP-1 Antibody

View all PARP1 Antibodies

SKU/Catalog Number

A00122-1

Size

100µl

Form

Liquid. Neat serum containing 0.1% sodium azide.

Description

Boster Bio Anti-PARP-1 Antibody catalog # A00122-1. Tested in ELISA, IP, IF, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

+4°C

Cite This Product

Anti-PARP-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00122-1)

Host

Rabbit

Contents

Liquid. Neat serum containing 0.1% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Recombinant human PARP-1 (poly (ADP-ribose) polymerase-1) (aa 1-1014) .

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00122-1 is reactive to PARP1 in Human, Mouse

Applications

A00122-1 is guaranteed for ELISA, IP, IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

113.084kDa

Background of PARP1

Participates in DNA repair and in chromosomal DNA replication.

Kesti T., J. Biol. Chem. 268:10238-10245(1993).
Syvaeoja J.E.;Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
Asahara H., Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry (1:4,000)
Immunoprecipitation (1:400)
Western Blot (1:4,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PARP1 using anti-PARP1 antibody (A00122-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP1 antigen affinity purified polyclonal antibody (Catalog # A00122-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for PARP1.

Gene/Protein Information For PARP1 (Source: Uniprot.org, NCBI)

Alternative Names

ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADPRT 1; ADPRT; ADPRTADP-ribosyltransferase NAD(+); EC 2.4.2; EC 2.4.2.30; NAD(+) ADP-ribosyltransferase 1; PARP apoptosis; PARP; PARP1; PARP-1; PARPADPRT1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL; PPOLpADPRT-1 PARP1 ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1 poly(ADP-ribose) polymerase 1 poly [ADP-ribose] polymerase 1|ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)|ADP-ribosyltransferase NAD(+)|ADP-ribosyltransferase diphtheria toxin-like 1|DNA ADP-ribosyltransferase PARP1|NAD(+) ADP-ribosyltransferase 1|poly (ADP-ribose) polymerase family, member 1|poly(ADP-ribose) synthetase|poly(ADP-ribosyl)transferase|poly[ADP-ribose] synthase 1|protein poly-ADP-ribosyltransferase PARP1

For more info on PARP1, check out the PARP1 Infographic

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In this infographic, you will see the following information for PARP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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