Anti-PCNA Antibody

PCNA antibody

Boster Bio Anti-PCNA Antibody (Catalog # M00125-2). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.

Product Info Summary

SKU: M00125-2
Size: 50 µl
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IHC-P, WB

Product Name

Anti-PCNA Antibody

View all PCNA Antibodies

SKU/Catalog Number

M00125-2

Size

50 µl

Description

Boster Bio Anti-PCNA Antibody (Catalog # M00125-2). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-PCNA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00125-2)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1655CT506.10.26

Isotype

IgG1,k

Immunogen

This PCNA antibody is generated from a mouse immunized with a recombinant protein of human PCNA.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00125-2 is reactive to PCNA in Human

Applications

M00125-2 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

28.769kDa

Background of PCNA

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000
IHC: 1:2000
IHC-P: 1:25
FC: 1:25

Validation Images & Assay Conditions

Gene/Protein Information For PCNA (Source: Uniprot.org, NCBI)

Gene Name

PCNA

Full Name

Proliferating cell nuclear antigen

Weight

28.769kDa

Superfamily

PCNA family

Alternative Names

cyclin; DNA polymerase delta auxiliary protein; MGC8367; proliferating cell nuclear antigen PCNA ATLD2 proliferating cell nuclear antigen proliferating cell nuclear antigen|DNA polymerase delta auxiliary protein|cyclin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PCNA, check out the PCNA Infographic

PCNA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PCNA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M00125-2-50ul

50 µl

$298
M00125-2-200ul

200 µl

$460

Free Secondary Antibody

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M00125-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$298.00

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